广西医科大学学报2021，Vol.38Issue(10)：1831-1840,10.DOI:10.16190/j.cnki.45-1211/r.2021.10.001
miR-203靶向ΔNp63对口腔鳞状细胞癌细胞增殖、侵袭、凋亡和干细胞特性的影响
Effects of miR-203 targeting ΔNp63 on proliferation, invasion, apoptosis and stem-cell characteristics of squamous cell carcinoma cells
摘要
Abstract
Objective:To investigate the effect of miR-203 tar-geting ΔNp63 on proliferation, invasion, apoptosis and stem-cell characteristics of oral squamous cell carcinoma (OSCC) cells. Methods: TCGA database was used to analyze the ex-pression of miR-203 and ΔNp63 in head and neck squamous cell carcinoma and adjacent normal tissues. RT-qPCR was used to detect the expression of miR-203 and ΔNp63 mRNA in OSCC cells (Tca8113, CAL-27, and SCC-9) and normal hu-man oral keratinocytes (NHOK). The CAL-27 cells were trans-fected with miR-NC, miR-203 mimic, pcDNA, or pcDNA- ΔNp63, and co-transfected with miR-203 mimic and pcDNA- ΔNp63. The expressions of ΔNp63, Bax, Bcl-2, Ki67, MMP-2, E-cadherin, N-cadherin, Lin28B, SOX2 and OCT4 were detect-ed by Western blotting. Luciferase reporter assay was used to analyze the targeting relationship between miR-203 and ΔNp63. CCK8, Transwell, and flow cytometry were performed respec-tively to detect cell viability, invasion, apoptosis and the num-ber of ALDH positive cells. A nude mouse xenograft tumor was constructed to detect the effect of miR-203 on tumors in vi-vo. Results: The expression of miR-203 was down-regulated while the expression of ΔNp63 was up-regulated in head and neck squamous cell carcinoma and OSCC cells (P<0.05). miR-203 targeted and negatively regulated the expression of ΔNp63. The expressions of ΔNp63, Ki67, MMP-2, N-cad-herin, Bcl-2, Lin28B, SOX2 and OCT4, the viability of CAL-27 cells, the number of invasive cells and ALDH-positive cells were decreased significantly, while the apoptosis rate and the protein expression levels of Bax and E-cadherin were increased in miR-203 group (P<0.01). The results of ΔNp63 group were opposite. miR-203 mimic reversed the promotive effects of overexpressed ΔNp63 on CAL-27 cell viability, invasion and stem cell-like properties (P<0.05). miR-203 inhibited tumor growth in nude mice translanted with OSCC cell line and downregulated ΔNp63 expression. Conclusion:miR-203 plays a tumor-suppressive role in OSCC by inhibiting the expression of ΔNp63.关键词
口腔鳞状细胞癌/miR-203/ΔNp63/侵袭/OCT4Key words
oral squamous cell carcinoma/miR-203/ΔNp63/invasion/OCT4分类
医药卫生引用本文复制引用
高婵,何爱娥,熊贵忠,谢霓,李民..miR-203靶向ΔNp63对口腔鳞状细胞癌细胞增殖、侵袭、凋亡和干细胞特性的影响[J].广西医科大学学报,2021,38(10):1831-1840,10.基金项目
This study was funded by Knowledge Innovation Project of Hubei Province in 2018(Natural Science Foundation of Hubei Province)(No.2018CFC834) （Natural Science Foundation of Hubei Province）
