遵义医科大学学报2021，Vol.44Issue(4)：433-439,7.
氟斑牙大鼠发生纤维瘤:内质网应激和线粒体的激活途径
Fibroma in dental fluorosis rats:the activated pathways of endoplasmic reticu-lum stress and mitochondria
摘要
Abstract
Objective To investigate whether rats were performed with dental fluorosis develop soft tissue tumors and the transcript levels of key genes in the endoplasmic reticulum stress and mitochondrial apoptosis pathways in tumor tissues. Methods A total of 20 SD rats were treated with sodium fluoride for 14 months. Rats were observed for their mental and other status as well as the presence or absence of tumors. The pathological structures of tumor tissues were tested by HE staining. RT-PCR was performed to detect the endoplasmic reticulum stress-related genes,such as IRE1α,PERK,eIF2α,ATF4,GRP78,GRP94,CHOP,XBP1 and ATF6 in the tumor tissues as well as transcript levels of genes related to the mitochondrial apoptosis pathway,including JNK, BCL2,Cyt-c,BAX, and Casp3. Results A soft tissue tumor was observed in the abdomen of one rat. with a size of (3. 5 × 4. 0) cm, which was intact in capsule,hard in texture and high in mobility. HE staining showed that the soft tissue tumors were composed of a large number of fibrous cells. Compared with adjacent tissues,the expressions of GRP78,GRP94,CHOP,IRE1α,PERK,eIF2α and ATF4 were all up-regulated in endoplasmic reticulum stress signaling pathway,while the expressions of XBP1 and ATF6 were down-regulated. In mitochondrial apoptosis pathway, BCL2,BAX,Cyt-c and JNK were up-regulated,while Casp3 was down-regulated. Conclusion SD rats with dental fluorosis can develop fibroma,and its molecular mechanism is related to the endoplasmic reticulum pathway and mitochondrial pathway,which might provide evidence for the tumorigenesis of sodium fluoride. However,more ex-perimental evidence is needed.关键词
氟斑牙/软组织肿瘤/纤维瘤/内质网应激/线粒体凋亡Key words
dental fluorosis/soft tissue tumor/fibroma/endoplasmic reticulum stress/mitochondrial apoptosis分类
医药卫生引用本文复制引用
曾蔼霖,李惠如,胡小艳,杨敏芝,母梅力,陈姚,刘建国,吴明松..氟斑牙大鼠发生纤维瘤:内质网应激和线粒体的激活途径[J].遵义医科大学学报,2021,44(4):433-439,7.基金项目
地方病与少数民族性疾病教育部重点实验室开放基金项目(NO:黔教合KY字[2017]384) （NO:黔教合KY字[2017]384）
贵州省普通高校口腔特色重点实验室开放课题(NO:K005) （NO:K005）
遵义市汇川区科技计划项目(NO:遵汇科合201632) （NO:遵汇科合201632）
遵义医科大学本科生创新创业培训计划(NO:201751059、20163737、2018521445、201710661041). （NO:201751059、20163737、2018521445、201710661041）
