生态科学2021，Vol.40Issue(5)：40-48,9.DOI:10.14108/j.cnki.1008-8873.2021.05.006
中草药粗提物杀灭离体鱾新本尼登虫效果
In vitro efficacy of crude plant extracts against Neobenedenia girellae
摘要
Abstract
Neobenedenia girellae is an ectoparasite of marine fishes, which causes major epidemics in marine aquaculture. In order to investigate the effect of salinity on the activity of the adults and oncomiracidia of N. girellae, the mortalities of N. girellae adults and oncomiracidia were evaluated at different salinities (0, 10, 20, and 30 ppt). Additionally, the in vitro efficacy of ethanol extracts of Polyporus umbellatus, Notopterygium incisum, Lophatherum gracile, Moutan cortex, and Arnebia euchroma against N. girellae was investigated. Results showed the adults and oncomiracidia of N. girellae showed 0.0％ mortality at 30 ppt of salinity after an 8 h and 5 h exposure at (22 ± 1) °C, respectively. They can maintain good activity power. The result provided time basis for the in vitro experiment of N. girellae. In vitro results indicated that N. incisum ethanol extract resulted in 100％ mortality of N. girellae adults at a concentration of 16 mg·L–1 within 158.7 min and killed all oncomiracidia at 8 mg·L–1 within 135.7 min. L. gracile, M. cortex, and A. euchroma extracts at ≥ 64 mg·L–1 killed all N. girellae oncomiracidia. P. umbellatus extract reduced adults and oncomiracidia survival. N. incisum extract at ≥ 128 mg·L–1 caused 0％ hatching rate with no egg development. However, when exposed to 256 mg·L–1 of P. umbellatus, L. gracile, and A. euchroma ethanol extract for 10 days, there was no significant efficacy on eggs hatching rate. These findings demonstrate that these plant extracts are potential sources of botanical drugs for controlling N. girellae infection in aquaculture.关键词
鱾新本尼登虫/植物提取物/抗寄生虫活性/活力Key words
Neobenedenia girellae/plant extract/antiparasitic efficacy/activity power分类
农业科技引用本文复制引用
刘彦甍,侯廷龙,付耀武,冯娟,张其中..中草药粗提物杀灭离体鱾新本尼登虫效果[J].生态科学,2021,40(5):40-48,9.基金项目
广东省省级科技计划项目(2016B090918112,2016B090918029) （2016B090918112,2016B090918029）
广东省渔业生态环境重点实验室开放基金(LFE-2016-2) （LFE-2016-2）
广州市科技计划项目(201604020040) （201604020040）
