昆虫学报2021，Vol.64Issue(10)：1205-1217,13.DOI:10.16380/j.kcxb.2021.10.010
基于线粒体基因组的华蝉族系统发育地位研究(半翅目:蝉科)
Phylogenetic status of the cicada tribe Sinosenini(Hemiptera:Cicadidae)based on mitochondrial genomes
摘要
Abstract
[Aim]The aim of this study is to clarify the phylogenetic status of the tribe Sinosenini in Cicadoidea,in which the cicadas have no timbal organs.[Methods]Based on adult specimens of Karenia caelatata of Sinosenini collected in Ningshan,Shaanxi,northwestern China,the mitochondrial genome of K.caelatata was sequenced,annotated,bioinformatically analyzed,and compared with the mitochondrial genomes of other taxa of Cicadoidea.Molecular phylogenetic trees for Cicadoidea were constructed using both maximum likelihood(ML)method and Bayesian inference(BI)method.[Results]The mitochondrial genome of K.caelatata(GenBank accession number:MN922304)is 14 960 bp in length,and the gene organization,nucleotide composition and codon usage of protein-coding genes display similar structural characteristics to those of other taxa of Cicadoidea.Analysis of nucleotide diversity reveals that genes atp8,nad6 and nad2 are highly variable,while cox 1 is more conserved.Ratios of nonsynonymous and synonymous substitution rates indicate that the evolution of mitochondrial genomes in Cicadoidea is under a high-level purifying selection.Results of phylogenetic analyses support the monophyly of Cicadomorpha,revealing the relationship of the three superfamilies of this infraorder as(Membraciodea+(Cicadoidea+Cercopodidea)).Karenia is clustered with representatives of the tribe Dundubiini in the subfamily Cicadinae and most closely related to the genus Meimuna.Mogannia and Vagitanus,representatives of Cicadatrini,are clustered with the members of Cicadettinae.Yezoterpnosia is not a monophyletic group.[Conclusion]Sinosenini should be transferred from Cicadettinae to Cicadinae and merged with Dundubiini,while Cicadatrini should be transferred from Cicadinae to Cicadettinae.Our results provide new information for future study of evolution of cicadas with different sound-producing mechanisms.关键词
蝉总科/蝉次目/鼓膜发音器/线粒体基因组/核苷酸多样性/进化速率Key words
Cicadoidea/Cicadomorpha/timbal organ/mitogenome/nucleotide diversity/evolutionary rate分类
生物科学引用本文复制引用
袁飞敏,魏琮..基于线粒体基因组的华蝉族系统发育地位研究(半翅目:蝉科)[J].昆虫学报,2021,64(10):1205-1217,13.基金项目
国家自然科学基金项目(31772505,32070476) （31772505,32070476）
