广西医科大学学报2021，Vol.38Issue(11)：2019-2027,9.DOI:10.16190/j.cnki.45-1211/r.2021.11.001
外泌体传递miR-145调控上皮性卵巢癌细胞转移及癌干细胞样特征研究
Exosomes deliver miR-145 to regulate epithelial ovarian cancer cell metastasis and cancer stem cell-like characteristics
摘要
Abstract
Objective:To investigate the effects of epithelial ovarian cancer (EOC) cell-secreted exosomal miR-145 on the proliferation,migration and invasion abilities and stem cell-like characteristics of EOC cells.Methods:Ovarian cancer SKOV3 cells were transfected with miR-145 mimic,miR-145 inhibitor,or miR-145 negative control (NC).Exosomes (Exo)derived from SKOV3 cells and miR-145-overexpressing or miR-145-downregulated SKOV3 cells were isolated and identified.The expression ofmiR-145 was assessed by RT-qPCR.SKOV3 cells were co-cultured with exosomes from different sources.Cell proliferation,migration and invasion were detected by MTT assay and Transwell assay.The impact of exosomal miR-145 on angiogenic tube formation ability of endothelial cells was tested by tube formation assay.The stem cell characteristics of CD133+/CD44+ cells sorted from SKOV3 cell line were evalu-ated by tumor sphere formation assay.Results:miR-145 ex-pression was significantly upregulated in SKOV3 cells treated with miR-145 mimic-Exo and downregulated in cells treated with miR-145 inhibitor-Exo.Exo derived from ovarian cancer cells favored the proliferation,migration and invasion of SKOV3 cells (P＜0.05).High levels of exosomal miR-145 decreased the proliferation,migration and proliferation of SKOV3 cells,inhibited angiogenesis in human umbilical vein endothelial cells,and suppressed tumor sphere forming capacity of SKVO3 cells (P＜0.05).However,miR-145-downregula-ted exosomes had opposite effects (P＜0.05).Conclusion:The transfer of miR-145 through exosomes can inhibit the migration,invasion and angiogenesis of epithelial ovarian cancer cells,and regu-late the stem cell-like characteristics of epithelial ovarian can-cer.关键词
卵巢癌上皮细胞/外泌体/miR-145/肿瘤干细胞Key words
epithelial ovarian cancer/exosome/miR-145/tu-mor stem cells分类
医药卫生引用本文复制引用
张文晶,王静,张玉瑾,余明莲,王辉..外泌体传递miR-145调控上皮性卵巢癌细胞转移及癌干细胞样特征研究[J].广西医科大学学报,2021,38(11):2019-2027,9.基金项目
This study was funded by Na-tional Natural Science Foundation of China (No.81341075). （No.81341075）
