水生生物学报2021，Vol.45Issue(6)：1201-1206,6.DOI:10.7541/2021.2020.294
唇䱻硬骨蛋白基因克隆及其在肌间刺发生过程中的表达
MOLECULAR CHARACTERIZATION OF A SCLEROSTIN HOMOLOGUE IN BARBEL STEED (HEMIBARBUS LABEO) AND ITS RELATIONSHIP WITH INTERMUSCULAR BONE DEVELOPMENT
摘要
Abstract
Previous studies have demonstrated that sclerostin (SOST) binds to and antagonizes the activity of bone morphogenetic proteins (BMPs). Because of the potential role of BMPs in intermuscular bone (IB) de-velopment, we explored the relationship between SOST and IB development. In this study, we identified a cDNA sequence encoding a SOST homologue in barbel steed (Hemibarbus labeo). Sequence analysis re-vealed that the SOST protein is composed of a signal peptide and a mature peptide which contained a C-ter-minal cystine knot-like domain. Phylogenetic tree analysis indicated that barbel steed SOST was closely re-lated to goldfish (Carassius auratus) SOST. Expression of SOST transcripts were detected in all the tested tissues, with the highest transcript levels being found in the gill. in situ RNA hybridization results showed that SOST mRNA was obviously distributed in the myosepta. RT-qPCR showed that the transcript levels of the SOST gene significantly changed during the four developmental stages of IBs. This timing overlapped with IB formation, providing support for a role of SOST in IB development.关键词
唇䱻/SOST/基因表达/肌间刺/骨化Key words
Barbel steed/SOST/Gene expression/Intermuscular bone/Ossification分类
生物科学引用本文复制引用
陈洁,刘子明,姜维,吕耀平..唇䱻硬骨蛋白基因克隆及其在肌间刺发生过程中的表达[J].水生生物学报,2021,45(6):1201-1206,6.基金项目
Supported by the Zhejiang Provincial Natural Science Foundation of China(Y3110477) （Y3110477）
the Research Project of Lishui Sci-ence and Technology Bureau(2018RC01) （2018RC01）
