昆虫学报2021，Vol.64Issue(11)：1244-1251,8.DOI:10.16380/j.kcxb.2021.11.002
利用全长转录本优化柞蚕基因组注释
Improvement of the annotation of Antheraea pernyi(Lepidoptera:Saturniidae) genome using full-length transcripts
摘要
Abstract
[Aim]This study aims to improve the annotation of the genome of the Chinese oak silkworm,Antheraea pernyi,so as to expand its application to comparative genomics and breed improvement.[Methods]The full-length transcriptome of A.pernyi was sequenced and analyzed,and compared with the reference genome to identify novel genes and transcripts with functional annotation and to predict long non-coding RNAs(lncRNAs).The gene models in the genome of A.pernyi were modified by thousands of novel protein-coding transcripts and lncRNAs.Finally,the corrected and merged annotation of the genome of A.pernyi was created.[Results]A total of 1997 novel protein-coding genes and 3399 novel lncRNA genes were discovered and supported by 2402 and 3574 full-length transcripts,respectively.The genome of A.pernyi contains 25021 genes,including 19825 protein-coding genes,from which seven juvenile hormone acid O-methyltransferase genes were identified.[Conclusion]This study improves our knowledge of the known genes in the genome of A.pernyi and provides valuable resources for comparative and functional genomic studies in A.pernyi and its relatives.关键词
柞蚕/基因组注释/全长转录组/蛋白编码基因/lncRNA/保幼激素酸甲基转移酶基因Key words
Antheraea pernyi/genome annotation/full-length transcriptome/protein-coding genes/lncRNA/juvenile hormone acid O-methyltransferase genes分类
生物科学引用本文复制引用
李莹,雷煜宇,梁世梅,章贤,杜杰,杨新峰,李闪闪,段建平..利用全长转录本优化柞蚕基因组注释[J].昆虫学报,2021,64(11):1244-1251,8.基金项目
河南省自然科学基金项目(182300410064) （182300410064）
河南省科技攻关项目(202102110200) （202102110200）
