实验动物与比较医学2021，Vol.41Issue(6)：528-534,7.DOI:10.12300/j.issn.1674-5817.2021.073
基于团体标准T/CALAS21—2017的Wistar大鼠微卫星DNA群体遗传质量分析
Population Genetic Quality Analysis of Microsatellite DNA in Wistar Rats Based on T/CALAS 21—2017
魏杰 1岳秉飞 1左琴 1王洪 1李欢 1周佳琪 1光姣娜 1范涛 1刘佐民 1付瑞1
作者信息
- 1. 中国食品药品检定研究院,北京102629
- 折叠
摘要
Abstract
Objective To analyze the genetic quality of an outbred stock of Wistar rats in different periods by T/CALAS 21—2017 method, and to evaluate the applicability of the association standard. Methods Wistar rats selected from the same outbred stock in 2015 and 2019 were named as group A and group B, respectively. Twenty-five pairs of microsatellite primers were used to get genetic parameters for quality analysis of the outbred stock Wistar rats according to the association standard T/CALAS 21—2017. Polymorphism information content (PIC) was used to analyze the polymorphism of the loci. Results One hundred alleles in group A and 69 alleles in group B were obtained. The average heterozygosity of the two groups was 0.574 and 0.447, while the average PIC was 0.541 and 0.393, respectively. Conclusion The association standard T/CALAS 21—2017 has good applicability in the genetic quality analysis of outbred stock rats, and the genetic diversity of group A is better than that of group B.关键词
Wistar大鼠/T/CALAS21-2017/封闭群/微卫星DNA/遗传多样性Key words
Wistar rats/T/CALAS 21—2017/Outbred stock/Microsatellite DNA/Genetic diversity分类
生物科学引用本文复制引用
魏杰,岳秉飞,左琴,王洪,李欢,周佳琪,光姣娜,范涛,刘佐民,付瑞..基于团体标准T/CALAS21—2017的Wistar大鼠微卫星DNA群体遗传质量分析[J].实验动物与比较医学,2021,41(6):528-534,7.
