东南大学学报（英文版）2021，Vol.37Issue(4)：388-393,6.DOI:10.3969/j.issn.1003-7985.2021.04.007
原油降解的功能微生物菌群的开发与性能分析
Development and characterization of a functional microbial consortium for crude oil degradation
摘要
Abstract
Crude oil-degrading microbial consortia were enriched from three oil-contaminated sites to achieve the efficient biodegradation of crude oil,especially its refractory residues.The gravimetric method was used to analyze the degradation efficiency of the enriched consortia and changes in the fractions of the crude oil.The effects of changes in environmental factors were also studied to determine the optimal oil-reducing conditions and assess the dominant bacteria of the mixed flora.Results show that all three consortia exhibit reliable crude oil-biodegradation abilities and that their mixture results in biodegradation rate are as high as(48.0 ± 3.5)％ over 30 d of incubation.The consortium mixture can degrade 11.1％ of the refractory resins,79.7％of the saturated hydrocarbons,and 45.7％ of the aromatics in crude oil.Neutral pH,an incubation temperature of 30 ℃,and low mineral salt concentrations (0.8％ to 4.0％) are optimal for crude oil biodegradation.The dominant genera in the consortium mixture include Pseudomonas,Stenotrophomonas,Brucella,Serratia,Brevundimonas,and Achromobacter.The richness and diversity of the microbial community in the consortium remain stable during crude oil degradation.Therefore,microbial enrichment from multiple sources may be performed to construct a mixed consortium for crude oil pollution bioremediation.关键词
原油/生物降解/微生物菌群/原油残渣Key words
crude oil/biodegradation/microbial consortium/refractory residues分类
资源环境引用本文复制引用
祝冲之,张晓东,孙丽伟,展漫军,龙涛,余冉..原油降解的功能微生物菌群的开发与性能分析[J].东南大学学报（英文版）,2021,37(4):388-393,6.基金项目
The National Natural Science Foundation of China(No.51878145),the National Key R&D Program of China (No.2018YFC1803100),the Key Research and Development Program of De-partment of Science and Technology of Jiangsu Province (No.BE2019709),the Six Talent Peaks Project of Jiangsu Province (No.JN-HB-010). （No.51878145）
