医学分子生物学杂志2022，Vol.19Issue(1)：1-9,9.DOI:10.3870/j.issn.1672-8009.2022.01.001
融合IL2的慢病毒实现对T细胞的高效基因转导
Potent Gene Transfer in Human Primary T Lymphocytes Using Interleukin-2-displaying Lentiviral Vectors
摘要
Abstract
Objective To construct the interleukin 2 (IL2) -displaying lentivirus to improve the transduction efficiency of human primary T lymphocytes. Methods The IL2 gene was fused to the N-terminus of vesicular stomatitis virus G protein to produce the novel lentivirus-packaging plas-mid pMD2.IL2-G. The yields of lentiviral particles pseudotyped with different doses of pMD2.IL2-G were compared using immunoblotting and ELISA. The infected capacity of IL2-displaying lentiviruses to T lymphocytes from healthy donors was tested using flow cytometry. Results The yield of lentivi-ral particles pseudotyped with pMD2.IL2-G was significantly less than that of wild-type lentiviruses packaged by pMD2.G. The mixture of pMD2.IL2-G and pMD2.G at a ratio of 1: 4 could obtain the equivalent yield of lentiviruses that of the wild-type, and significantly improve the transduction effi-ciency of enhanced green fluorescent protein into the T lymphocytes compared to the wild-type lenti-viruses (89.2 ％vs.40.2 ％). Conclusion Our results demonstrate that IL2-displaying lentiviral particles have a greatly enhanced transduction efficiency into human T lymphocytes. This research is expected to provide strong support for the clinical application of genetically modified T cells.关键词
T细胞/包膜蛋白/融合白介素-2的慢病毒载体/转导效率/过继免疫疗法Key words
T lymphocytes/envelope glycoprotein/interleukin-2-displaying lentiviruses/transduction efficiency/adoptive immunotherapy分类
生物科学引用本文复制引用
王依依,杜晓,胡志嵩,刘欣禹,欧阳清,赵晶,杨安钢,阎博..融合IL2的慢病毒实现对T细胞的高效基因转导[J].医学分子生物学杂志,2022,19(1):1-9,9.基金项目
国家自然科学基金重点项目(No.81630069),国家自然科学基金面上项目(No.81972870) This work was supported by grants from the Key Project of the National Natural Science Foundation of China(No.81630069)and the National Natural Science Foundation of China(No.81972870) （No.81630069）
