数字中医药（英文）2021，Vol.4Issue(4)：305-315,11.DOI:10.1016/j.dcmed.2021.12.005
急性脑梗死患者血清外泌体差异基因表达谱的研究
Study on differential gene expression profile of serum exosomes in patients with acute cerebral infarction
摘要
Abstract
Objective To analyze the differential gene expression profile of serum exosomes in patients with acute cerebral infarction (ACI) and clarify the changes in gene expression related to cerebral in-farction injury and the potential serum markers.Methods Four patients with ACI and five healthy people were en-rolled in the Phase Ⅰ study. After serum isolation from peripheral blood, exosomes were extracted with exosomes kits, high-throughput detection of mRNA was performed with gene chips, and differentially expressed mRNAs were screened. Gene Onto-logy (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were per-formed simultaneously. Furthermore, real-time polymerase chain reaction (qRT-PCR) was used to verify the expression levels of the screened differential mRNAs in the serum exosomes collec-ted in Phase Ⅱ from 32 patients each in the ACI case and normal control groups. Results In the Phase Ⅰ study, there were 248 differentially ex-pressed mRNAs (fold change ≥ 2.0, P < 0.05) among five pa-tients in the normal control group and four patients in the case group, of which the expression of 242 was upregulated and that of six was downregulated. The results of GO functional enrichment analysis mainly included behavior regulation, cell connection, and antioxidant activity. The results of KEGG pathway enrich-ment analysis mainly included ribosomes, proteasomes, oxyto-cin signaling pathways, and oxidative phosphorylation. After re-searching and screening based on relevant literature, it was found that among the genes with significant differential expression, H3F3B mRNA may be associated with and might play an important role in ACI. The qRT-PCR method was used to detect the H3F3B mRNA expression in serum exosomes of 32 patients each in the normal control and case groups in Phase Ⅱ; the expression was significantly higher in serum exosomes of the case group than in those of the normal control group (P < 0.001). H3F3B mRNA expression in serum exosomes of the case group positively correlated with age, the National Institutes of Health Stroke Scale (NIHSS) score, and the maximum infarct size (P <0.05).Conclusion ACI can lead to changes in the serum exosomes mRNA expression profile, which may be closely related to the oc-currence, development, and prognosis of this condition. These findings will provide direction for research on the molecular mechanism, diagnostic markers, and therapeutic targets of ACI.关键词
急性脑梗死/外泌体/mRNA/表达谱/基因芯片/H3F3BKey words
Acute cerebral infarction (ACI)/Exosomes/mRNA/Expression profile/Gene chip/H3F3B引用本文复制引用
唐荣梅,陈博威,易健,刘柏炎,林华山..急性脑梗死患者血清外泌体差异基因表达谱的研究[J].数字中医药（英文）,2021,4(4):305-315,11.基金项目
We thank for the funding support from the National Natural Science Foundation of China(No.82074251),the Hunan Natural Science Foundation of China(No.2018JJ2413),and the Hunan Provincial Health and Health Commission Project(No.c2018032). （No.82074251）
