数字中医药（英文）2021，Vol.4Issue(4)：316-327,12.DOI:10.1016/j.dcmed.2021.12.006
妇科千金方对子宫肌瘤模型大鼠激素及其受体的影响及代谢组学研究
Effects of Fuke Qianjin Formula on hormones and their receptors and metabonomics study in uterine fibroids model rats
摘要
Abstract
Objective To investigate the effects of different fractions from Fuke Qianjin Formula (妇科千金方,FKQJF) on uterine leiomyoma (UL) to determine the best fraction.Methods FKQJF was extracted and isolated to obtain polysac-charides (FKP), flavonoids (FKF), and grease (FKG). 140 female SPF SD rats were divided into 14 groups [model (MOD), normal control (NC), Gouliuqing (GLQ), Mifepristone (MFST), FKQJF, low, medium, and high dose of polysaccharides (l-FKP, m-FKP, and h-FKP), low, medium, and high dose of flavonoids (l-FKF, m-FKF, and h-FKF), low, medium, and high dose of grease (l-FKG, m-FKG, and h-FKG)], and uterine fibroids model rats were treated with drugs for four weeks. Serum levels of estrogen and progesterone were measured using enzyme-linked immun-oassay assay (ELISA) kits. The expression of estrogen receptor (ER-α, ER-β) and progesterone receptor (PR) in the uterus was observed using immunohistochemistry (IHC). Serum metabolite profiles and FKG were analyzed using gas chromatography-mass spectrometry (GC-MS). Results FKQJF, h-FKF, m-FKG, and h-FKG significantly down-regulated the estrogen level in the uterine fibroid model rats (P < 0.01). FKQJF, h-FKF, and h-FKG significantly reduced the level of progesterone in the uterine fibroid model rats (P < 0.01). The levels of ER-α, ER-β, and PR in uterine fibroid model rats were significantly decreased by FKQJF and h-FKG (P <0.01). The levels of ER-α, ER-β, and PR in the fibroid model rats were de-creased by m-FKG (P < 0.05). Additionally, serum metabolism results revealed that h-FKG and FKQJF could regulate related en-dogenous metabolites and make the pathological indices of uter-ine fibroids in rats close to the normal group. Forty-six compon-ents were identified in the oil, accounting for 91.97％ of the total oil components.Conclusion FKQJF and h-FKG showed a significant anti-myoma activity and significantly improved the pathological state of the uterus in rats with hysteromyoma. The mechanism of action may be related to the regulation of estrogen progesterone and its re-ceptor in uterine fibroid model animals. These findings proved the effect of FKQJF on uterine leiomyoma and provided an exper-imental basis for its clinical research and application.关键词
妇科千金方/子宫肌瘤/油脂/气相色谱法/代谢组学/雌激素/孕激素/雌激素受体/孕激素受体Key words
Fuke Qianjin Formula (妇科千金方, FKQJF) Uterine leiomyoma/Grease/Gas chromatography-mass spectrometry (GC-MS)/Metabolomics/Estrogen/Progesterone/Estrogen receptor/Progesterone receptor引用本文复制引用
李亚梅,唐洁,罗弘杉,夏伯候,林丽美,廖端芳..妇科千金方对子宫肌瘤模型大鼠激素及其受体的影响及代谢组学研究[J].数字中医药（英文）,2021,4(4):316-327,12.基金项目
We thank for the funding support from the Major Sci-ence and Technology Projects in Hunan Province(No.2015SK1001),Special Funds from the Central Government to Guide Local Science and Technology Development(No.2019XF5076),Natural Science Foundation of Hunan Province(No.2017JJ1023 and No.2019JJ50443),and the Research project of Hunan Education Department(No.17B198 and No.19C1398).We thank Drs.GONG Yun and ZHANG Peng of Zhuzhou Qianjin Pharmaceutical Co.,Ltd.for their valuable suggestions during the entire research pro-cess. （No.2015SK1001）
