赣南医学院学报2021，Vol.41Issue(12)：1193-1202,10.DOI:10.3969/j.issn.1001-5779.2021.12.001
染料木素通过调节能量代谢和抑制氧化应激缓解大鼠运动性疲劳的研究
Genistein in alleviating rat sports fatigue by regulating energy metabolism and inhibiting oxidative stress
摘要
Abstract
Objective:To evaluate the potential beneficial effects and the underlying mechanism of Gen on the sports fatigue. Methods: Male SD rats were randomly and uniformly divided into four groups(control,vehicle,0. 1 μmol·kg-1 Gen,and 0. 3 μmol·kg-1 Gen),and sports fatigue was induced by swimming exercise. Thirty minutes before swimming, normal saline was injected subcutaneously into the control group and vehicle group. The treatment group was subcutaneously injected with 0. 1 μmol·kg-1 Gen,and 0. 3 μmol·kg-1 Gen,The control group was not subjected to swimming exercise. The vehicle group and the treatment group were forced to swim once a day for 14 days. The potential anti-fatigue functions induced by Gen,and the underlying mechanism were evaluated after the last exhaustive swimming by determining the levels of blood glucose,muscle glycogen,hepatic glycogen,Total antioxidant capacity (T-AOC),Glutathione peroxidase (GSH-Px),Superoxide dismutase(SOD),and Malondialdehyde(MDA). The determination was done about the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in both muscle and liver,and the activities of lactate dehydrogenase(LDH)and creatine kinase(CK)in the quadriceps femoris muscle. Results: Gen increased the consumption of blood glucose as well as the levels of muscle glycogen and hepatic glycogen. It also increased the serum T-AOC,GSH-PX and SOD but decreased the serum MDA. In addition,gen increased the activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase,LDH and CK. Conclusion:Gen could protect against sports fatigue by regulating energy metabolism and maintaining the free radical and antioxidant system balance.关键词
染料木素/运动疲劳/能量代谢/抗氧化系统Key words
Genistein/Sports fatigue/Energy metabolism/Antioxidant system分类
医药卫生引用本文复制引用
麻海娟,曾雪亮,李贝,黎晓,吴丽珍,李良东,黄诚,黄志华..染料木素通过调节能量代谢和抑制氧化应激缓解大鼠运动性疲劳的研究[J].赣南医学院学报,2021,41(12):1193-1202,10.基金项目
National Natural Science Foundation of China(No.31360250) （No.31360250）
