广西医科大学学报2021，Vol.38Issue(12)：2199-2207,9.DOI:10.16190/j.cnki.45-1211/r.2021.12.001
基于网络药理学的左归丸治疗骨质疏松作用机制探讨
Study on the mechanism of Zuogui pill in treating osteoporosis based on network pharmacology
摘要
Abstract
Objective:To explore the effective active ingredi-ents,targets and potential molecular mechanisms of Zuogui pill in the treatment of osteoporosis by means of network phar-macology.Methods:Using the TCMSP database to predict the active ingredients of Zuogui pill,and to screen out the poten-tial active ingredients and targets.The disease target genes re-lated to osteoporosis were retrieved from the GeneCards data-base.After screening through the Drugbank and Uniprot data-bases,the Zuogui pill-osteoporosis disease intersection genes were finally obtained.Cytoscape 3.8.2 software was used to construct a drug component-disease network diagram,and the intersection genes were used to construct a protein interaction network,and the core network and core target genes of Zuogui pill treatment for osteoporosis were obtained,and the core tar-get genes were analyzed by using MCODE and Metascape da-tabases.GO bio-enrichment analysis and KEGG pathway anal-ysis were performed on the relevant targets through the Metascape database.Finally,cell experiments were used to fur-ther verify the effects of Zuogui pill on osteoclasts in vitro.Re-sults:A total of 245 effective targets of Zuogui Pill and 4,576 targets of osteoporosis-related diseases are predicted,and there are 171 common targets.The active ingredients with higher moderate values are quercetin,kaempferol,isorhamnetin,β-si-tosterol,and 24-methylenephenol.The core genes in the PPI network are APP,NTRK1,EGFR,CUL3,and HSP90AA1.GO enrichment analysis involves DNA binding transcription factor binding,RNA polymerase Ⅱ specific DNA binding tran-scription factor binding and nuclear receptor activity;KEGG pathway enrichment analysis results show that the common tar-gets can pass through the AGE-RAGE signaling pathway,TNF signaling pathway,IL-17 signal pathway,HIF-1 signal path-way and many other signal pathways.The cell experiments show that Zuogui pill could inhibit the formation of osteoclasts In vitro.Conclusion:Zuogui pill may play a role in the preven-tion and treatment of osteoporosis by inhibiting osteoclast for-mation,providing a new thinking route and method for follow-up basic experimental research.关键词
左归丸/骨质疏松/网络药理学Key words
Zuogui pill/osteoporosis/network pharmacology分类
医药卫生引用本文复制引用
李晶,吴立蔚,许杨,肖承祥,周波..基于网络药理学的左归丸治疗骨质疏松作用机制探讨[J].广西医科大学学报,2021,38(12):2199-2207,9.基金项目
This study was funded by Nation-al Natural Science Foundation of China(No.81860655) （No.81860655）
