农业生物技术学报2022，Vol.30Issue(4)：641-655,15.DOI:10.3969/j.issn.1674-7968.2022.04.003
辣椒CaPIP5K基因家族的鉴定与特异性表达分析
Identification and Specific Expression Analysis of CaPIP5K Gene Family in Pepper (Capsicum annuum)
摘要
Abstract
Phosphatidylinositol signaling system plays an important role in plant development. Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is one of the most crucial enzyme that catalyze PIP2 synthesis in this signaling system. The present study identified 19 CaPIP5K family genes in pepper (Capsicum annuum) genome database, among which 14 CaPIP5Ks were dispersedly distributed on 8 out of the 12 chromosomes and 5 CaPIP5Ks located on chromosome 0. Except 1 PIP5K domain was confirmed in every CaPIP5K proteins, 5～8 repeated MORN (membrane occupation and recognition nexus) domains were also detected in 8 CaPIP5Ks. All CaPIP5K proteins were predicted to locate in the cytoplasm. Moreover, cis-acting elements in CaPIP5K promoter region mainly responded to hormones, defense and stress, and low temperature. In addition, the expressional analysis indicated that CaPIP5K4-1 not only specifically expressed in flower instead of other tissues, but also gradually improved with the development of flower buds in fertile accessions as compared to sterile accession. This study provides a reference basis for further exploring the mechanism of CaPIP5K gene family in the development of anther and pollen in pepper.关键词
辣椒/磷酸脂酰肌醇-4-磷酸-5-激酶(PIP5K)/基因家族/雄性育性/表达分析Key words
Pepper/Phosphatidylinositol 4-phosphate 5-kinase (PIP5K)/Gene family/Male fertility/Expression analysis分类
农业科技引用本文复制引用
王永富,赵淑芳,苟秉调,段盼盼,魏敏,杨楠,张高原,魏兵强..辣椒CaPIP5K基因家族的鉴定与特异性表达分析[J].农业生物技术学报,2022,30(4):641-655,15.基金项目
国家自然科学基金(31760572) （31760572）
甘肃农业大学青年导师扶持基金(GAU-QDFC-2020-07) （GAU-QDFC-2020-07）
甘肃省高校创新基金(2020B-128) （2020B-128）
