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Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding: A case study of estuarine fish diversity monitoring

Hui-Ting Ruan Rui-Li Wang Hong-Ting Li Li Liu Tian-Xu Kuang Min Li Ke-Shu Zou

动物学研究(英文)2022，Vol.43Issue(2)：P.192-204,13.

动物学研究(英文)2022，Vol.43Issue(2)：P.192-204,13.DOI:10.24272/j.issn.2095-8137.2021.331

Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding: A case study of estuarine fish diversity monitoring

Hui-Ting Ruan ¹Rui-Li Wang ²Hong-Ting Li ³Li Liu ²Tian-Xu Kuang ²Min Li ⁴Ke-Shu Zou²

作者信息

1. University Joint Laboratory of Guangdong Province,Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation,College of Marine Science,South China Agricultural University,Guangzhou,Guangdong 510642,China Guangzhou CAOMUFAN Ecological Research limited company,Guangzhou,Guangdong 510520,China
2. University Joint Laboratory of Guangdong Province,Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation,College of Marine Science,South China Agricultural University,Guangzhou,Guangdong 510642,China
3. Key Laboratory for Sustainable Utilization of Open-sea Fishery,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510300,China College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China
4. Key Laboratory for Sustainable Utilization of Open-sea Fishery,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510300,China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou,Guangdong 511458,China
折叠

摘要

关键词

eDNA metabarcoding/Fish diversity/Sampling strategies/DNA extraction/Estuarine ecosystem

分类

生物科学

引用本文复制引用

Hui-Ting Ruan,Rui-Li Wang,Hong-Ting Li,Li Liu,Tian-Xu Kuang,Min Li,Ke-Shu Zou..Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding: A case study of estuarine fish diversity monitoring[J].动物学研究(英文),2022,43(2):P.192-204,13.

基金项目

supported by the National Natural Science Foundation of China(32102793) （32102793）

National Key R&D Program of China(2018YFD0900802) （2018YFD0900802）

Central Public-Interest Scientific Institution Basal Research Fund （）

South China Sea Fisheries Research Institute,CAFS(2019TS13,2021SD18) （2019TS13,2021SD18）

Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0605) （Guangzhou）

Open Fund Project of Key Laboratory of Offshore Fishery Development of Ministry of Agriculture and Rural Affairs(LOF 2020-02) （LOF 2020-02）

China-ASEAN Maritime Cooperation Fund(CAMC-2018F)。（CAMC-2018F）

动物学研究(英文)

OACSCDCSTPCD

ISSN：2095-8137

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