数字中医药（英文）2022，Vol.5Issue(1)：75-82,8.DOI:10.1016/j.dcmed.2022.03.008
参远苷抑制脂多糖诱导的BV2小胶质细胞炎症机制研究
The therapeutic mechanism of Shenyuan Gan in lipopolysaccharide-induced neuroinflammation in BV2 microglial cells
摘要
Abstract
Objective To study the therapeutic effects of Shenyuan Gan (参远苷, SYG) on the inflammat-ory response in BV2 microglial cells induced by lipopolysaccharide (LPS).Methods The cytotoxicity of SYG to BV2 microglial cells was evaluated using a Cell Counting Kit-8 (CCK-8) assay, and the effect of SYG concentrations on LPS-induced BV2 microglial cells was studied. The morphological changes were observed using an optical microscope. The nitric oxide (NO) concentration in cell culture supernatant was determined using Griess re-agent. The expression of cytokines and inflammatory mediators were also measured by an en-zyme-linked immunosorbent assay (ELISA). Western blot analysis was used to determine the levels of inducible NO synthase (iNOS), nuclear factor-kappa B (NF-κB) p65, alpha inhibitor of NF-κB (IκB-α), phosphorylation-IκB-α (p-IκB-α), NOD-like receptor 3 (NLRP3), and cas-pase-1 expression. Moreover, the expression of iNOS, NLRP3, and ionized calcium binding adapter molecule 1 (Iba1) was also observed using immunofluorescent staining. Results SYG had a low cytotoxic effect on BV2 microglial cells and could significantly decr-ease LPS-induced morphological changes of BV2 microglial cells (P < 0.05). ELISA results showed that SYG significantly inhibited the LPS-induced increase in interleukin (IL)-1β and IL-6 in BV2 microglia cells (P < 0.05), and Western blot analysis showed that the phosphoryla-tion levels of iNOS, NF-κB p65, and IκB-α as well as NLRP3 and caspase-1 expression were also significantly decreased, and IκB-α expression was increased after SYG treatment (P <0.05, compared with the LPS-treated group). The immunofluorescence results were consist-ent with the Western blot results, and Iba1 staining indicated that the cell morphology tended to be resting. These results indicate that SYG has a certain inhibitory effect on LPS-induced inflammation in BV2 microglial cells. Conclusion SYG can inhibit LPS-induced release of inflammatory factors in BV2 microglial cells by affecting the phosphorylation levels of NF-κB p65 and IκB-α. SYG is a valuable candid-ate for treating neuroinflammation-related diseases.关键词
参远苷/神经炎症/促炎因子/BV2小胶质细胞/脂多糖Key words
Shenyuan Gan (参远苷, SYG)/Neuroinflammation/Pro-inflammatory mediators/BV2 microglial cells/Lipopolysaccharide (LPS)引用本文复制引用
彭莎,彭壮,胡秦,刘新民,陈颖,石哲..参远苷抑制脂多糖诱导的BV2小胶质细胞炎症机制研究[J].数字中医药（英文）,2022,5(1):75-82,8.基金项目
The Space Medical Experiment Project of the China Manned Space Program(HYZHXM05003),National  （HYZHXM05003）
Natural Science Foundation of China(82171493),Natur-al Science Foundation of Hunan province(2021JJ30504),Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A04905),and Scientific Research Fund of Hunan Provincial Education of the Hunan University of Tradi-tional Chinese Medicine First-class Discipline Project of Chinese Medicine(19B422). （82171493）
