分析测试学报2022，Vol.41Issue(4)：509-519,11.DOI:10.19969/j.fxcsxb.22010201
RNA中腺嘌呤编辑分析方法研究进展
Research Progress on Analytical Methods for De?ciphering Adenosine-to-inosine RNA Editing
摘要
Abstract
Adenosine-to-inosine(Ade-to-Ino)RNA editing is one of the most widespread post-transcriptional modifications in RNA. The conversion of adenosine to inosine is through the hydrolytic deamination of the amino group at the C6 position of adenosine, which is catalyzed by the adenosine deaminases acting on RNA(ADARs). Accumulated lines of evidence show that Ade-to-Ino RNA editing is involved in the regulation of gene expression and protein functions.Aberrant Ade-to-Ino RNA editing is demonstrated to be correlated with many human diseases.In-depth investigation of the biological functions of Ade-to-Ino RNA editing depends on the sensitive detection,accurate quantification, and precise mapping analysis. This review provides an overview of the recent advances in analytical methods and techniques for the detection, quantification and location analysis of Ade-to-Ino RNA editing in a wide range of RNA molecules. The principles, advantages, limitations and applications of these established methods are discussed. It is hoped that this review could stimulate the development of analytical methods to decipher Ade-to-Ino RNA editing and expedite the elucidation of the functions of Ade-to-Ino RNA editing in various RNA species .关键词
RNA腺嘌呤编辑/RNA修饰/检测方法/定位分析Key words
adenosine-to-inosine RNA editing/RNA modification/detection method/mapping analysis分类
化学化工引用本文复制引用
陈娟娟,袁必锋,冯钰锜..RNA中腺嘌呤编辑分析方法研究进展[J].分析测试学报,2022,41(4):509-519,11.基金项目
Foundation item:National Natural Science Foundation of China(22074110,21635006,21721005) （22074110,21635006,21721005）
