分析测试学报2022，Vol.41Issue(4)：520-528,9.DOI:10.19969/j.fxcsxb.21121601
基于CRISPR/Cas的生物传感平台在分子诊断中的应用
CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis
摘要
Abstract
Clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated proteins (Cas)have become highly promising candidates in the construction of biosensing systems and diagnostic devices,in addition to their roles as revolutionary genome engineering tools.For invitrodiagnostics,the classⅡ CRISPR/Cas systems possess unique advantages, such as high detection sensitivity and specificity, and the feasibility for developing point-of-care diagnostic technology. This minireview briefly introduces the working mechanism of CRISPR/Cas systems and mainly focus on the various diagnostic methods based on the CRISPR/Cas for detecting different forms of the genetic molecules in recent three years according to different readout methods .关键词
CRISPR/Cas/分子诊断/即时检测/生物传感Key words
CRISPR/Cas/molecular diagnosis/point-of-care testing/biosensing分类
化学化工引用本文复制引用
李甜,李娜..基于CRISPR/Cas的生物传感平台在分子诊断中的应用[J].分析测试学报,2022,41(4):520-528,9.基金项目
Foundation item:Natural Science Foundation of Henan Province of China(202300410145) （202300410145）
National Natural Science Foundation of China(22135004,21974006) （22135004,21974006）
