中南民族大学学报（自然科学版）2022，Vol.41Issue(4)：406-412,7.DOI:10.12130/znmdzk.20220404
虾青素对体外脂多糖诱导下结直肠癌发生的影响
Effects of astaxanthin against colorectal carcinogenesis by lipopolysaccharide challenge in vitro
摘要
Abstract
To evaluate the chemoprotective ability of astaxanthin(ASTA)against colorectal carcinogenesis after lipopolysaccharide (LPS)challenge in HCT116 cells. IL-6 and IL-1βsecretion,apoptosis,caspase-3 and anti-caspase-9,as well as NF-κB and Nrf2 transference into nuclear,and mRNA expression of NF-κB and Nrf2 were investigated in HCT116 cells with or without ASTA treatment after LPS challenge. The results indicated that LPS challenge significantly increased IL-1βand IL-6 secretion in HCT 116 cells,while the secretion was remarkably inhibited by ASTA in a concentration-dependent manner. ASTA increased the percentage of apoptotic cells,increased expression of caspase-9 and caspase-3,upregulated mRNA expression of Nrf2 and downregulated mRNA expressions of NF-κB in HCT116 cells. ASTA prevented the initiation and development of colorectal cancer(CRC)and regulated NF-κB and Nrf2 signaling pathways in HCT116 cells. These findings suggested NF-κB and Nrf2 signaling pathways might play a critical role for ASTA's prevention of CRC,and ASTA might be a new and attractive chemoprevention agent for inflammation-related cancer.关键词
虾青素/直肠癌/脂多糖/NF-κB/Nrf2Key words
astaxanthin/colorectal cancer/lipopolysaccharide/NF-κB/Nrf2分类
医药卫生引用本文复制引用
汪文俊,王新宇,黄文伟,田薇,曾国祥..虾青素对体外脂多糖诱导下结直肠癌发生的影响[J].中南民族大学学报（自然科学版）,2022,41(4):406-412,7.基金项目
湖北省自然科学基金资助项目(2016CFB487) （2016CFB487）
中央高校基本科研业务费专项资金资助项目(CZZ16003) （CZZ16003）
