分析测试学报2022，Vol.41Issue(8)：1138-1145,8.DOI:10.19969/j.fxcsxb.21123002
超临界流体色谱法快速定量分析吴茱萸中的吴茱萸碱和吴茱萸次碱
Rapid Quantification of Evodiamine and Rutae?carpine in Evodia Rutaecarpa(Juss.)Benth. Using Supercritical Fluid Chromatography
摘要
Abstract
This study proved the feasibility of using supercritical fluid chromatography(SFC)in rapid quantification of evodiamine and rutaecarpine in Evodia rutaecarpa(Juss. )Benth.(E. rutaecarpa). The two alkaloids were firstly ex?tracted by supercritical fluid extraction(SFE). As a result, the developed SFE conditions were as follows, sample (120-200 mesh)/cellulose acetate=1:1, 20％ethanol as modifier, 40℃, 25 MPa and the CO2 flow rate of 8 mL/min.The developed SFC method was carried out on a 2-EP column,with methanol as modifier at a flow rate of 1. 4 mL/min, a column temperature of 35℃and a back pressure of 15 MPa. The baseline separation of evodiamine and rutaecarpine was completed within 6 min.After verification,the SFC method was confirmed to be linear with correla?tion coefficients(r2) of 0. 9998, good precision(RSD below 0. 50％) and accuracy(recoveries of 102％-109％). Meanwhile,limits of detection(LODs)and limits of quantitation(LOQs)for evodiamine were 1. 00 and 3. 33μg/mL, while LOD and LOQ for rutaecarpine were 0. 95 and 3. 17μg/mL,respectively.Finally,the method was applied to the determination of contents of evodiamine and rutaecarpine in ten E. rutaecarpa samples from 4 areas. The total content of target alkaloids accounted 2. 95％for Guangdong, 1, 0. 51％for Guangdong, 2, 1. 27％for Guizhou, 3, 1. 00％for Hunan,4,0. 93％for Hunan,5,1. 81％for Jiangxi,6,0. 73％for Jiangxi,7,0. 58％for Jiangxi,8, 0. 41％for Jiangxi,9 and 0. 36％for Jiangxi,10.Although the ten samples all meet the requirements for the Chinese Pharmacopoeia, the origin difference of sample quality is very obvious, and the samples from the same place also have differences. In addition, the developed method was compared with the Pharmacopoeia method(2020 Edition). The results of content determination by the two methods were similar(1. 27％by SFC vs.1. 12％by Pharmacopoeia), while the time required by SFC(6 min)was significantly shorter than that by the Pharmacopoeia method(25 min). This work showed the potential of SFC in the fast quantification of active components in traditional Chinese medicines .关键词
超临界流体色谱/超临界流体萃取/定量/吴茱萸/吴茱萸碱/吴茱萸次碱Key words
supercritical fluid chromatography(SFC)/supercritical fluid extraction(SFE)/quantification/Evodia rutaecarpa(Juss. )Benth./evodiamine/rutaecarpine分类
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白国玉,戴莹萍,祝赵静韬,李开伦,姜文佳,徐冬月,傅青,金郁..超临界流体色谱法快速定量分析吴茱萸中的吴茱萸碱和吴茱萸次碱[J].分析测试学报,2022,41(8):1138-1145,8.基金项目
The Undergraduate Training Program on Innovation and Entrepreneurship grant X20115 （）
