南方医科大学学报2022，Vol.42Issue(10)：1431-1439,9.DOI:10.12122/j.issn.1673-4254.2022.10.01
SOX2-OT/SOX2轴通过Gli1介导的上皮间质转化调控肺鳞癌H520细胞的迁移
SOX2-OT/SOX2 axis regulates lung cancer H520 cell migration via Gli1-mediated epithelial-mesenchymal transition
摘要
Abstract
Objective To explore the regulatory role of SOX2-OT in migration of lung squamous cell carcinoma H520 cells and the underlying mechanisms. Methods Wound-healing and Transwell migration assays were performed to examine the changes in migration and invasion capacity of lung squamous cell line H520, which expressed higher levels of SOX2-OT than other lung cancer cell lines, following RNA interference-mediated SOX2-OT knockdown. The transcription levels of epithelial-mesenchymal transition (EMT)-related components was detected by qRT-PCR and immunoblotting. Gli1 gain-of-function analysis was performed in H520 cells with SOX2-OT knockdown and the changes in EMT phenotype of the cells were examined. miR-200c mimic and inhibitor were used to analyze the mechanism by which SOX2-OT positively regulates Gli1 and the mediating role of SOX2. Results SOX2-OT knockdown significantly lowered the invasiveness and migration capacity of H520 cells and caused changes in EMT phenotype of the cells. Overexpression of Gli1, which was positively regulated by SOX2-OT, reversed the inhibitory effect of SOX2-OT knockdown on migration of H520 cells. Transfection of the cells with miR-200c inhibitor effectively reversed SOX2-OT knockdown-induced down-regulation of SOX2. Conclusion The SOX2-OT/SOX2 axis positively regulates migration of lung squamous H520 cells via Gli1-mediated EMT.关键词
SOX2-OT/H520细胞/Gli1/SOX2/上皮间质转化Key words
SOX2-OT/lung squamous cell carcinoma/Gli1/SOX2/epithelial-mesenchymal transition引用本文复制引用
董洪亮,曾莉莉,武艳,苗双,倪娜,刘乃国,陈微微,杜静..SOX2-OT/SOX2轴通过Gli1介导的上皮间质转化调控肺鳞癌H520细胞的迁移[J].南方医科大学学报,2022,42(10):1431-1439,9.基金项目
Supported by National Natural Science Foundation of China(31900441,81903102),Natural Science Foundation of Shandong Province(ZR2019MC026),Shandong Medical and Health Technology Development Program(2017WS155,2019WS321),and Shandong Traditional Chinese Medicine Science and Technology Project(2019-0514)国家自然科学基金(31900441,81903102) （31900441,81903102）
山东省自然科学基金(ZR2019MC026) （ZR2019MC026）
山东省医疗卫生技术发展计划项目(2017WS155、2019WS321) （2017WS155、2019WS321）
山东省中医药科技发展项目基金(2019-0514) （2019-0514）
