中南民族大学学报（自然科学版）2023，Vol.42Issue(1)：42-46,5.DOI:10.20056/j.cnki.ZNMDZK.20230106
高温影响拟南芥减数分裂染色质上5-甲基胞嘧啶的分布
High temperatures alter distribution of 5-methylcytosine on chromatin in Arabidopsis meiosis
摘要
Abstract
In many plant species,it has been evidenced that both generation and distribution of DSBs and COs are shaped by DNA methylation status of the chromatin. In Arabidopsis,elevation of environmental temperature over the tolerable range of DSB formation system influences CO distribution,and/or reduces CO formation by interfering with generation of DSBs and the assembly of synaptonemal complex. However, whether epigenetic modifications especially for DNA methylation of the chromosomes have an impact on response of MR to high temperatures remain unknown. To this end,a preliminary analysis of the distribution of 5-methylcytosine (5mC) was performed on prophase chromosomes in Arabidopsis plants incubated at high temperatures. Immunostaining using an anti-5mC antibody showed that under control temperature,5mC signals were dominantly located at heterochromatin regions where chromosomes showed a high staining florescence by 4',6-diamidino-2-phenylindole(DAPI). In contrast,under higher temperatures at both 32℃and 36-38℃, distribution of 5mC florescence is reshaped at different chromatin regions,suggesting that DNA methylation landscape be altered under the increased temperatures. This study therefore hinted that elevation of environmental temperatures may affect DSB and/or CO patterning by modifying DNA methylation status.关键词
拟南芥/减数分裂/同源重组/高温胁迫/DNA双链断裂Key words
Arabidopsis/meiosis/meiotic recombination/DNA methylation/DSB分类
农业科技引用本文复制引用
刘虹,杨珂,宁莹洁,马月平,宋粲,杨杰峰..高温影响拟南芥减数分裂染色质上5-甲基胞嘧啶的分布[J].中南民族大学学报（自然科学版）,2023,42(1):42-46,5.基金项目
湖北省科技创新条件共享平台资助项目(2021DFE021) （2021DFE021）
