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结构光照明显微镜重建算法研究进展

周博 王昆浩 陈良怡

中国光学2022,Vol.15Issue(6):1211-1227,17.
中国光学2022,Vol.15Issue(6):1211-1227,17.DOI:10.37188/CO.EN.2022-0011

结构光照明显微镜重建算法研究进展

Recent progress on the reconstruction algorithms of structured illumination microscopy

周博 1王昆浩 2陈良怡1

作者信息

  • 1. 北京大学未来技术学院分子医学研究所,北大-清华生命科学联合中心,膜生物学国家重点实验室,心脏代谢分子医学北京市重点实验室,北京100871
  • 2. 华南师范大学生物光子学院,激光生命科学教育部重点实验室,广东广州510631
  • 折叠

摘要

Abstract

As an early component of modern Super-Resolution(SR)imaging technology,Structured Illumin-ation Microscopy(SIM)has been developed for nearly twenty years.With up to~60 nm wavelengths and 564 Hz frame rates,it has recently achieved an optimal combination of spatiotemporal resolution in live cells.Despite these advantages,SIM also suffers disadvantages,some of which originated from the intrinsic recon-struction process.Here we review recent technical advances in SIM,including SR reconstruction,perform-ance evaluation,and its integration with other technologies to provide a practical guide for biologists.

关键词

结构光照明显微镜/超分辨率成像

Key words

structured illumination microscopy/super-resolution imaging

分类

数理科学

引用本文复制引用

周博,王昆浩,陈良怡..结构光照明显微镜重建算法研究进展[J].中国光学,2022,15(6):1211-1227,17.

基金项目

国家自然科学基金项目(No.81925022,No.92054301,No.91750203,No.31821091) (No.81925022,No.92054301,No.91750203,No.31821091)

国家重点研究发展计划U项目(No.SQ2016YFJC040028) (No.SQ2016YFJC040028)

北京市自然科学基金项目(No.Z200017,No.Z201100008420005,No.Z20J00059) (No.Z200017,No.Z201100008420005,No.Z20J00059)

国家科技重大专项(No.2016YFA0500400)Supported by National Natural Science Foundation of China(No.81925022,No.92054301,No.91750203,No.31821091) (No.2016YFA0500400)

National Key R&D Program of China(No.SQ2016YFJC040028) (No.SQ2016YFJC040028)

Beijing Natural Science Found-ation(No.Z200017,No.Z201100008420005,No.Z20J00059) (No.Z200017,No.Z201100008420005,No.Z20J00059)

National Science and Technology Major Project Programme(No.2016YFA0500400) (No.2016YFA0500400)

中国光学

OA北大核心CSCDCSTPCD

2095-1531

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