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An optimized protocol using Steedman's wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flowerbuds of cucumber

WANG Cui SUN Jin-jing YANG Xue-yong WAN Li ZHANG Zhong-hua ZHANG Hui-min

农业科学学报（英文）2023，Vol.22Issue(2)：464-470,7.

农业科学学报（英文）2023，Vol.22Issue(2)：464-470,7.DOI:10.1016/j.jia.2022.08.038

An optimized protocol using Steedman's wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flowerbuds of cucumber

WANG Cui ¹SUN Jin-jing ²YANG Xue-yong ²WAN Li ²ZHANG Zhong-hua ¹ZHANG Hui-min¹

作者信息

1. College of Horticulture,Qingdao Agricultural University/Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province/Laboratory of Quality&Safety Risk Assessment for Fruit(Qingdao),Ministry of Agriculture and Rural Affairs,Qingdao 266109,P.R.China
2. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs/Sino-Dutch Joint Laboratory of Horticultural Genomics,Beijing 100081,P.R.China
折叠

摘要

关键词

cucumber/in situ hybridization/Steedman's wax/paraffn wax

Key words

cucumber/in situ hybridization/Steedman's wax/paraffn wax

引用本文复制引用

WANG Cui,SUN Jin-jing,YANG Xue-yong,WAN Li,ZHANG Zhong-hua,ZHANG Hui-min..An optimized protocol using Steedman's wax for high-sensitivity RNA in situ hybridization in shoot apical meristems and flowerbuds of cucumber[J].农业科学学报（英文）,2023,22(2):464-470,7.

基金项目

We thank Dr.Hu Bowen(Hunan Agricultural University,China)for valuable suggestions on this program.This work was supported by the National Natural Science Foundation of China(32002036). （Hunan Agricultural University,China）

农业科学学报（英文）

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ISSN：2095-3119

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