遵义医科大学学报2023，Vol.46Issue(2)：105-113,9.
肾细胞癌细胞中PBRM1-SWI/SNF复合体通过E2F1参与细胞周期控制
PBRM1-SWI/SNF complex contributes to cell cycle control via E2F1 in renal cell carcinoma cells
摘要
Abstract
Objective The aim of this study is to investigate whether PBRM1 deficiency affects cell proliferation and how this process is regulated at the transcriptional level.Methods We performed RNA interference,RT-PCR,Western blot,MTT,and FACS experiments to analyze cell proliferation and expression of E2F1 family mem-bers after PBRM1 knockdown.Meanwhile,the transcription regulation mechanism of E2F1 and PBRM1 was also investigated by the ChIP experiment.Results Our data showed that PBRM1 deficiency in SN12C cells significant-ly increased the mitotic index (MI ),which was accompanied by upregulation of E2F1 transcription factors. PBRM1 was recruited to the E2F1 promoter,co-occurring with active histone modifications.E2F1 was recruited to the PBRM1 promoter and could play a repressive role in PBRM1 expression via binding to its response elements. Conclusion These data suggest that the PBRM1-containing SWI/SNF complex plays an antiproliferative role.De-ficiency of PBRM1 along with downstream deregulated genes by PBRM1 and/or E2F members may act synergisti-cally in ccRCC carcinogenesis and development.关键词
ccRCC/PBRM1/E2F1转录因子/SWI/SNF/细胞周期/组蛋白修饰Key words
ccRCC/PBRM1/E2F1 transcription factor/SWI/SNF/cell cycle/histone modification分类
医药卫生引用本文复制引用
潘有福,金燕红,李胡丽,辛慧,田瑜,徐丹,郑前,张晓兰..肾细胞癌细胞中PBRM1-SWI/SNF复合体通过E2F1参与细胞周期控制[J].遵义医科大学学报,2023,46(2):105-113,9.基金项目
国家自然科学基金资助项目(NO:31760321) （NO:31760321）
贵州省科技厅基金资助项目(NO:[2017]1217,[2018]1189) （NO:[2017]1217,[2018]1189）
遵义医科大学博士启动基金资助项目(NO:F-972). （NO:F-972）
