无机材料学报2023，Vol.38Issue(1)：55-61,中插1,8.DOI:10.15541/jim20220119
普鲁士蓝基HClO荧光纳米探针及其对肿瘤细胞的特异性检测
Construction of Prussian Blue Fluorescent Nanoprobe for Specific Detection of HClO in Cancer Cells
摘要
Abstract
Hypochlorous acid (HClO) is one of the reactive oxygen species (ROS), taking crucial parts in many physiological and pathological processes. However, excessive HClO causes tissue injuries, atherosclerosis, neurodegeneration diseases, and even cancers. Therefore, real-time detection of HClO in cancer cells is of importance for exploring the effect of HClO in tumor progression or immunotherapy. Quite different from present organic molecular probes, a novel inorganic-based hydrophilic fluorescent nanoprobe was developed by simply integrating fluorescein isothiocyanate (FITC) into hollow mesoporous Prussian Blue nanoparticles (HMPB) in this work. Owing to inner filter effect, fluorescence of FITC within HMPB quenches to some extent, which can be restored via the Fe2+-ClO– redox reaction. A typical fluorescence increase of FITC at emission peak of 520 nm can be clearly observed in the presence of HClO in vitro, which exhibits a good linear relationship in the range of 5×10–6–50×10–6 mol/L in HClO detection and its detection limit is calculated to be 2.01×10–6 mol/L. Furthermore, the cellular experiment demonstrates the specific detection capability of HClO in cancer cells with high sensitivity by the obtained nanoprobe.关键词
普鲁士蓝/荧光探针/HClO检测Key words
Prussian Blue/fluorescent probe/detection of HClO分类
化学化工引用本文复制引用
杜邱静,刘天智,陈菊锋,陈航榕..普鲁士蓝基HClO荧光纳米探针及其对肿瘤细胞的特异性检测[J].无机材料学报,2023,38(1):55-61,中插1,8.基金项目
National Key R&D Program of China(2021YFB3801001) （2021YFB3801001）
National Natural Science Foundation of China(32030061) （32030061）
The Key Program for Basic Research of Shanghai(19JC1415600). （19JC1415600）
