南方医科大学学报2023，Vol.43Issue(2)：157-165,9.DOI:10.12122/j.issn.1673-4254.2023.02.01
体外重建宫颈癌的三维培养模型:基于人宫颈癌组织的去细胞支架
A cervical cancer tissue-derived decellularized extracellular matrix scaffold for cervical cancer tissue reconstruction in vitro
摘要
Abstract
Objective The prepare decellularized extracellular matrix (ECM) scaffold materials derived from human cervical carcinoma tissues for 3D culture of cervical carcinoma cells. Methods Fresh human cervical carcinoma tissues were treated with sodium lauryl ether sulfate (SLES) solution to prepare decellularized ECM scaffolds. The scaffolds were examined for ECM microstructure and residual contents of key ECM components (collagen, glycosaminoglycan, and elastin) and genetic materials by pathological staining and biochemical content analysis. In vitro 3D culture models were established by injecting cultured cervical cancer cells into the prepared ECM scaffolds. The cells in the recellularized scaffolds were compared with those in a conventional 2D culture system for cell behaviors including migration, proliferation and epithelial-mesenchymal transition (EMT) wsing HE staining, immunohistochemical staining and molecular biological technology analysis. Resistance to 5-fluorouracil (5-Fu) of the cells in the two culture systems was tested by analyzing the cell apoptosis rates via flow cytometry. Results SLES treatment effectively removed cells and genetic materials from human cervical carcinoma tissues but well preserved the microenvironment structure and biological activity of ECM. Compared with the 2D culture system, the 3D culture models significantly promoted proliferation, migration, EMT and 5-Fu resistance of human cervical cancer cells. Conclusion The decellularized ECM scaffolds prepared using human cervical carcinoma tissues provide the basis for construction of in vitro 3D culture models for human cervical cancer cells.关键词
细胞外基质/去细胞支架/宫颈癌/肿瘤微环境Key words
extracellular matrix/decellularized scaffolds/cervical carcinoma/tumor microenvironments引用本文复制引用
毛建英,杨文静,郭和,董瑞丽,任丽芳,李树斌..体外重建宫颈癌的三维培养模型:基于人宫颈癌组织的去细胞支架[J].南方医科大学学报,2023,43(2):157-165,9.基金项目
Supported by Natural Science Foundation of Inner Mongolia Autonomous Region(2019MS08098) （2019MS08098）
Science and Technology Research Project of Inner Mongolia Autonomous Region(201702118) （201702118）
Research Foundation Project of Inner Mongolia People's Hospital(2021YN12).内蒙古自治区自然科学基金(2019MS08098) （2021YN12）
内蒙古自治区科技计划项目(201702118) （201702118）
内蒙古自治区人民医院院内基金(2021YN12) （2021YN12）
