深圳大学学报（理工版）2023，Vol.40Issue(2)：163-170,8.DOI:10.3724/SP.J.1249.2023.02163
ShRNA-LDH纳米颗粒的制备及薇甘菊生物防治
Preparation of ShRNA-LDH nanoparticle and its application in biological control of Mikania micrantha
摘要
Abstract
Ribonucleic acid interference (RNAi) is a highly efficient, safe, and environmentally friendly method in plant protection. Exogenous spraying of short hairpin RNAs (shRNAs) targeting light-harvesting chlorophyll a or chlorophyll b binding protein genes onto the leaves of Mikania micrantha (M. micrantha) H. B. K. can efficiently silence the expression of these genes. To improve the effectiveness of shRNA-mediated control of M. micrantha, layered double hydroxide (LDH) clay nanosheets with a diameter between 10 to 100 nm and good biocompatibility and environmental friendliness were prepared as shRNA carriers in this study. We found that the optimal binding mass ratio of LDH to shRNA was 1:10. ShRNA spraying assays showed that M. micrantha leaves with micro-wound were more sensitive to shRNA, and spraying LDH directly to M. micrantha had no effect on plant growth. To compare the effects of LDH-shRNA and shRNA alone on the control of M. micrantha, we sprayed LDH loaded with six different shRNA respectively or shRNA alone onto M. micrantha leaves, and a more severe necrotic phenotype was observed on the 6th day after LDH-shRNA-sprayed. Taken together, our study successfully developed an LDH-based shRNA carrying system to significantly improve the effectiveness of RNAi in the control of M. micrantha.关键词
核糖核酸干扰/植物保护/薇甘菊/叶绿素a/b结合蛋白/黏土纳米片/短发夹核糖核酸Key words
RNA interference/plant protection/Mikania micrantha/light harvesting chlorophyl a/b binding protein/clay nanosheets/short hairpin RNA分类
农业科技引用本文复制引用
莫亦琳,陈伟钊,黄丽娟,吴飞燕,肖念,于宇,刘学东..ShRNA-LDH纳米颗粒的制备及薇甘菊生物防治[J].深圳大学学报（理工版）,2023,40(2):163-170,8.基金项目
National Key R&D Program of China(2019YFA0903902) （2019YFA0903902）
National Natural Science Foundation of China(32270595) （32270595）
Shenzhen Science and Technology Program(JCYJ20190808115005598)国家重点研发计划资助项目(2019YFA0903902) （JCYJ20190808115005598）
国家自然科学基金资助项目(32270595) （32270595）
深圳市基础研究计划资助项目(JCYJ20190808115005598) （JCYJ20190808115005598）
