农业生物技术学报2023，Vol.31Issue(5)：914-926,13.DOI:10.3969/j.issn.1674-7968.2023.05.003
CaMV 35S增强子调节玉米基因表达的机制研究
Study on the Mechanism of CaMV 35S Enhancer Regulating Gene Expression in Maize (Zea mays)
摘要
Abstract
Maize (Zea mays) is an important food crop, feed source and industrial raw material. With the development of bio-technology, transgenic and other new technologies are widely used in maize breeding. Cauliflower mosaic virus 35S (CaMV 35S) promoter and its enhancer are widely used in transgenic operation of maize and other crops. Therefore, studying the mechanism of CaMV 35S enhancer has important theoretical value for future application. Two trait mutants were screened from a maize activation-tagging mutant library previously constructed by the research group on genetic improvement of maize. Based on the identification of artificial transposon insertion sites in the chromosomes, the spatio-temporal variation of gene RNA expression in the range of about 100 kb upstream and downstream of the transposon insertion sites was analyzed using qPCR. The results showed that the enhancer of CaMV 35S significantly up-regulated the gene expression of adjacent genes (P<0.05), and the transcriptionally increased genes were distributed upstream and downstream of the transposon insertion site, with activation distance of up to 63.7 kb. Of the 4 genes up-regulated expression, the enhancement was dose-dependent, and the expression of the homozygous mutant was significantly higher than that of the heterozygous mutant (P<0.05). Although the CaMV 35S enhancer increased the transcription level of genes in the maize mutants, it did not significantly change the expression pattern of genes in different tissues. The scope, dosage effect, and tissue specificity of CaMV 35S enhancer in maize provide an experimental basis for subsequent use in maize molecular biology, and genetic engineering, and can also provide reference for the application of the promoter in other plants.关键词
玉米/CaMV 35S增强子/CaMV 35S启动子/基因表达特征Key words
Maize/CaMV 35S enhancer/CaMV 35S promoter/Gene expression characteristics分类
农业科技引用本文复制引用
郭金洁,张丹,张伟,张方东,杜邓襄..CaMV 35S增强子调节玉米基因表达的机制研究[J].农业生物技术学报,2023,31(5):914-926,13.基金项目
国家自然科学基金(31871636 （）
31271737) （）
国家自然科学技术重大专项(2014ZX08003003 （）
2016ZX08003003) （）
