日用化学工业（中英文）2023，Vol.53Issue(4)：390-397,8.DOI:10.3969/j.issn.1001-1803.2023.04.004
吡罗克酮乙醇胺盐的蛋白酶抑制作用及与硫酸锌复配的防脱发功效研究
Novel role of piroctone olamine on protease inhibition in vitro, and reduction in human hair shedding in combination of zinc sulfate in vivo
Ranjit Kaur Bhogal 1陈旭斌 2David Messenger 1宫哲慧 2孙佳凝 2皮瑛瑛 2顾佳音2
作者信息
- 1. 联合利华科沃斯研发中心 人类生物科学与技术,贝德福德郡 MK441LQ,英国
- 2. 联合利华上海研发中心 美容健康事业部,上海 200335
- 折叠
摘要
Abstract
The process leading to the final step of hair shedding is reported to be driven by proteolytic activity and contributes to the detachment of club hair. During late telogen and exogen, increased protease activity may lead to excessive and unwanted hair loss. Materials that can correct proteolytic activity could be beneficial in reducing excessive hair loss. Piroctone olamine was assessed for the protease inhibition efficacy in vitro by deploying a direct fluorescence-based assay for detecting metallo-, serine, acid and sulfhydryl proteases. The assay detects protease activity by the means of a labelled pH-insensitive fluorescent dye where an increase in fluorescence is proportional to protease activity. The assay allows the assessment of materials that may inhibit protease activity of the target enzymes. Since serine proteases have been shown to play a role in the exogen phase of the hair growth cycle, trypsin a known serine protease was used as the target enzyme. Piroctone olamine was found to inhibit trypsin protease activity in a dose-dependent manner. The inhibitory effect was boosted with the addition zinc sulfate. The combination of zinc sulfate with piroctone olamine was formulated into a shampoo base for the evaluation of hair density efficacy in vivo. Male and female participants with hair loss concerns treated their scalp with a shampoo wash (treatment or placebo) 3 times per week for 12 weeks. Hair density was assessed at baseline followed by 4 weekly intervals. The results showed a significant increase in hair density following shampoo treatment versus baseline and versus placebo suggesting the combination of piroctone olamine and zinc sulfate plays a role in retaining hairs and consequently reducing hair fall.关键词
脱发/脱落期/毛发密度/蛋白酶/吡罗克酮乙醇胺盐/硫酸锌Key words
hair shedding/exogen/hair density/protease/piroctone olamine/zinc sulfate分类
化学化工引用本文复制引用
Ranjit Kaur Bhogal,陈旭斌,David Messenger,宫哲慧,孙佳凝,皮瑛瑛,顾佳音..吡罗克酮乙醇胺盐的蛋白酶抑制作用及与硫酸锌复配的防脱发功效研究[J].日用化学工业（中英文）,2023,53(4):390-397,8.
