数字中医药（英文）2023，Vol.6Issue(1)：73-85,13.DOI:10.1016/j.dcmed.2023.02.009
金银花和山银花9种抗炎成分对二甲苯耳肿胀模型小鼠体内的多成分药代动力学比较研究
Comparative polypharmacokinetics of nine anti-inflammatory components of Jinyinhua(Lonicerae Japonicae Flos)and Shanyinhua(Lonicerae Flos)in mice with p-xylene-induced ear edema
摘要
Abstract
Objective To reveal the integral in vivo polypharmacokinetics(PPK)similarity or difference between Jinyinhua(Lonicerae Japonicae Flos,LJF)and Shanyinhua(Lonicerae Flos,LF),and provide reference for their clinical application.Methods The PPK model and its total quantum statistical moment similarity(TQSMS)meth-od were used to compare the integral PPK profiles of nine components with anti-inflammat-ory efficacy(rutin,caffeic acid,chlorogenic acid,cryptochlorogenic acid,dispsacoside B,macranthoidin B,isochlorogenic acid A,isochlorogenic acid B,and isochlorogenic acid C)of LJF and LF.A total of 54 Specific Pathogen Free(SPF)grade Kunming(KM)mice were ran-domized into LJF group and LF group(n = 27),and each group was divided into nine sub-groups(n =3)according to different time points.Subsequently,mice model of p-xylene-in-duced ear edema was constructed by oral administration of LJF and LF.The concentrations of the nine anti-inflammatory components in plasma samples of the mice were determined by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry(UP-LC-QTOF-MS/MS).And the pharmacokinetics(PK)parameters of single component and the integral PPK parameters[total quantum statistical moment(TQSM)and TQSMS]of multiple components were calculated by Drug And Statistics(DAS)software and home-brew pro-grams with Excel,respectively.Results There were significant differences in single-component PK parameters between LJF and LF(P<0.05).Whereas,no significant differences were found in multi-component TQSM parameters,including total quantum zero moment(AUCT0-t,AUCT0-∞)and total quantum first moment(MRTT0-t,MRTT0-∞)for the total quanta(P>0.05).Accordingly,single-component TQSMS varied from 0.2204 to 0.9689,and that for the total quanta was 0.8284,suggesting no significant differences in the speed and extent of bioavailability between LJF and LF.Further-more,in light of high TQSMS(0.8284),the integral PPK profiles of the nine anti-inflammat-ory components of LJF and LF were similar under 90％confidence intervals.Conclusion The PPK model and its TQSMS method are appropriate and efficient to compare the similarity or difference of integral PPK profiles of multi-component herbal medicines. It is suggested in this research that LJF can be replaced with LF or vice versa for anti-inflammat-ory treatment.关键词
金银花/山银花/多成分药代动力学模型/总量统计矩相似度/抗炎作用/一致性Key words
Jinyinhua(Lonicerae Japonicae Flos,LJF)/Shanyinhua(Lonicerae Flos,LF)/Polypharmacokinetics(PPK)model/Total quantum statistical moment simil-arity(TQSMS)method/Anti-inflammatory efficacy/Consistency引用本文复制引用
李海英,肖美凤,潘雪,李文姣,周逸群,刘文龙,贺福元..金银花和山银花9种抗炎成分对二甲苯耳肿胀模型小鼠体内的多成分药代动力学比较研究[J].数字中医药（英文）,2023,6(1):73-85,13.基金项目
National Natural Science Foundation of China(81703824),Natural Science Foundation of Hunan Province(2021JJ80058 and 2021JJ30509),Research Fund and Joint Fund Project of Hunan University of Chinese Medicine(2021XJJJ002),Postgraduate Teaching Platform Project of Hunan Province(Xiang Jiao Tong[2019]No.334),and Postgraduate Research Innovation Program of Hunan Province(CX20210689). （81703824）
