中山大学学报（自然科学版）（中英文）2023，Vol.62Issue(3)：89-99,11.DOI:10.13471/j.cnki.acta.snus.2022E027
傣药"雅解":宾蒿、傣百解及竹叶兰的生物活性及其解毒机制
Biological activities and detoxification mechanisms of Clerodendrum chinense var.simplex,Marsdenia tenacissima and Arundina graminifolia:The Dai antidotes
摘要
Abstract
Dai antidotes are the most distinctive medicine and treatment in traditional Dai medicine.Bin Hao(Clerodendrum chinense var.simplex),Dai Bai Jie(Marsdenia tenacissima)and Zhu Ye Lan(Arun-dina graminifolia)are three Dai antidotes widely used for their"detoxifying effects",and their use is rooted in a theoretical system significantly different and much less understood than Western or tradi-tional Chinese medicines.Here,we successively extracted the three Dai antidotes using petroleum ether,ethyl acetate,n-butanol,or water,and then prepared their decoctions.The content of total flavo-noids in three Dai antidotes ranged from 22.41 to 586.39 mg/g,which is higher than the content of total polyphenols(2.76 to 28.66 mg/g).The various extracts were found to scavenge radicals of DPPH,·OH and ABTS.They scavenged ABTS radicals much more efficiently than other radicals(IC50>380 μg/mL).They weakly inhibited the growth of E.coli,P.aeruginosa and S.aureus.Notably,even at low concentra-tion 60 μg/mL,the extracts can significantly down-regulate the production of NO,TNF-α,IL-1β,and IL-6 by macrophages stimulated with LPS.In conclusion,our results provide the first mechanistic insights into the detoxifying effects of three Dai antidotes,providing a foundation for their optimization and for future research to strengthen Dai medicine through modern scientific practices.关键词
傣药"雅解"/宾蒿/傣百解/竹叶兰/解毒作用Key words
Dai antidote/Clerodendrum chinense var.simplex/Marsdenia tenacissima/Arundina graminifolia/detoxification分类
医药卫生引用本文复制引用
张雪飞,赵俊可,郝素旗,丁青,于世慧,伊木朗,王军,胡海燕..傣药"雅解":宾蒿、傣百解及竹叶兰的生物活性及其解毒机制[J].中山大学学报（自然科学版）（中英文）,2023,62(3):89-99,11.基金项目
Supported by Yunnan Provincial Department of Education Science Research Fund Project(2018JS709) （2018JS709）
National Natural Science Foundation of China(81973264) （81973264）
Guangdong Basic and Applied Basic Research Foundation(2019A1515011954,2020A1515010593) （2019A1515011954,2020A1515010593）
