渔业研究2023，Vol.45Issue(3)：213-221,9.DOI:10.14012/j.cnki.fjsc.2023.03.001
盐源山溪鲵转录组组装与分析
Transcriptome data assembly and analysis of Batrachuperus yenyuanensis
摘要
Abstract
The genomic information and transcriptome sequences of Batrachuperus yenyuanensis are rarely reportedat present. In this study, the transcripts of this species were sequenced and assembled using Illumin a HiSeqplatform. A total of 36 252 unigenes were obtained through sequencing and assembling, with an averagelength of 937 by and a N50 of 1 549 bp. Additionally, GC content, length distribution and expression level of unigenes were assessed. The result showed that the sequencing data was of high quality. Blaste d againstthe Swiss-prot, Nr, Pfam, KEGG, KOG and GO, a total of 16 465, 18 749, 13 983,10 607, 15 704 and 14 242 unigenes were annotated, respectively. According to the functional annotation results, 17 472coding sequence(CDS) were detected. A total of 2 779 microsatellite markers(SSR) and 3 100 sin gle nu-cleotide polymorphisms(SNP) were identified. KEGG pathway analysis showed that 10 607 unigen es wereannotated, and it was divided into 257 categories according to the different pathways. Among all the path-ways，the number of unigenes involved in signal transduction pathway exhibited the largest proportion. In thisstudy，the obtained transcriptome sequences provided rich data resources for further understanding of geneticdiversity analysis, molecular marker development, population resource evaluation and conservation, as wellas functional gene cloning of the B. yenyuanensis.关键词
盐源山溪鲵/转录组/unigenes/基因注释Key words
Batrachuperus yenyuanensis/transcriptome/unigenes/gene annotation分类
农业科技引用本文复制引用
朱文文,郁川,熊建利,黄勇..盐源山溪鲵转录组组装与分析[J].渔业研究,2023,45(3):213-221,9.基金项目
国家自然科学基金项目(31471971) （31471971）
河南省高等学校青年骨干教师资助计划项目(2015GGJS-054) （2015GGJS-054）
