Major royal-jelly proteins intake modulates immune functions and gut microbiota in miceOACSTPCD
In this study,we investigated the effects of major royal jelly proteins(MRJPs)on the estrogen,gut microbiota,and immunological responses in mice.Mice given 250 or 500 mg/kg,not 125 mg/kg of MRJPs,enhanced the proliferation of splenocytes in response to mitogens.The splenocytes and mesenteric lymphocytes activated by T-cell mitogens(Con A and anti-CD3/CD28 antibodies)released high levels of IL-2 but low levels of IFN-γand IL-17A.The release of IL-4 was unaffected by MRJPs.Additionally,splenocytes and mesenteric lymphocytes activated by LPS were prevented by MRJPs at the same dose as that required for producing IL-1βand IL-6,two pro-inflammatory cytokines.The production of IL-1β,IL-6,and IFN-γwas negatively associated with estrogen levels,which were higher in the MRJP-treated animals than in the control group.Analysis of the gut microbiota revealed that feeding mice 250 mg/kg of MRJPs maintained the stability of the natural intestinal microflora of mice.Additionally,the LEf Se analysis identified biomarkers in the MRJP-treated mice,including Prevotella,Bacillales,Enterobacteriales,Gammaproteobacteria,Candidatus_Arthromitus,and Shigella.Our results showed that MRJPs are important components of royal jelly that modulate host immunity and hormone levels and help maintain gut microbiota stability.
Hang Wu;Shican Zhou;Wenjuan Ning;Xiao Wu;Xiaoxiao Xu;Zejin Liu;Wenhua Liu;Kun Liu;Lirong Shen;Junpeng Wang;
Infection and Immunity Institute and Translational Medical Center of Huaihe Hospital,Henan University,Kaifeng 475000,ChinaCollege of Biology Science and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061,ChinaCollege of Biosystems Engineering and Food Science,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang University,Hangzhou 310058,China
轻工业
Major royal-jelly proteinsImmunityEstrogenGut microbiotaCytokines
《食品科学与人类健康(英文)》 2024 (001)
P.444-453 / 10
financially supported by the National Natural Science Foundation of China(U2004104);the Natural Science Foundation of Henan Province(202300410080);the Key Project of Henan Education Committee(21A310005);the Internal Fund of Hebei University of Economics and Business(2020ZD10);the Postgraduate“Talent Program”of Henan University(SYL20060187 and SYL20060189)。
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