花生学报2023，Vol.52Issue(2)：52-60,81,10.DOI:10.14001/j.issn.1002-4093.2023.02.007
伯克氏菌对群结腐霉引起的花生果腐病的生防潜力
Biocontrol Potentiality of Endophytic Burkholderia cepacia against Peanut Pod Rot Caused by Pythium myriotylum
摘要
Abstract
Peanut pod rot caused by Pythium myriotylum results in significant economic losses worldwide during peanut production.Biological control is an alternative measure to current chemical control methods,which are often inefficient and have adverse environmental effects.To control this disease,39 endophytic bacterial strains from healthy peanut(Arachis hypogaea)roots were screened for their antagonistic activi-ties against P.myriotylum.Five of which showed efficient biocontrol effects.Results showed,they were all identified as Burkholderia cepacia on the basis of 16S ribosomal RNA and Rec A gene sequences.A-mong the five B.cepacia strains,PRI08 manifested the greatest antagonistic activity against P.myrioty-lum mycelial growth.In greenhouse and field experiments,infected peanut plants with B.cepacia PRI08 inoculation reduced the disease incidence by 29.5％and 11.83％,respectively.Moreover,B.cepacia PRI08 significantly promoted peanut growth by enhancing the main stem length,lateral branch length,pod number,plump pod rate and pod yield by 20.44％,19.98％,26.61％,20.36％and 21.52％,respectively.This study revealed that B.cepacia PRI08 has the potential to be used as an agricultur-al biocontrol agent and plant growth-promoting bacterium.关键词
群结腐霉/内生菌/伯克氏菌/生物防治Key words
Pythium myriotylum/endophyte/Burkholderia bacteria/biological control分类
农业科技引用本文复制引用
于静,宋新颖,李莹,何康,郭志青,许曼琳,张霞,迟玉成..伯克氏菌对群结腐霉引起的花生果腐病的生防潜力[J].花生学报,2023,52(2):52-60,81,10.基金项目
国家自然科学基金项目(32202326) （32202326）
山东省自然科学基金项目(ZR2020QC131) （ZR2020QC131）
