南方医科大学学报2023，Vol.43Issue(6)：873-878,6.DOI:10.12122/j.issn.1673-4254.2023.06.01
蛹虫草代谢产物虫草素抑制舌鳞癌裸鼠移植瘤的生长
Cordycepin,a metabolite of Cordyceps militaris,inhibits xenograft tumor growth of tongue squamous cell carcinoma in nude mice
摘要
Abstract
Objective To evaluate the inhibitory effect of cordycepin on oral cancer xenograft in nude mice and explore the underlying mechanisms.Methods Sixteen BALB/c mice bearing subcutaneous human tongue squamous cell carcinoma(TSCC)TCA-8113 cell xenografts were randomized into model group and cordycepin treatment group for daily treatment with saline and cordycepin for 4 weeks.After the treatment,the tumor xenografts were dissected and weighed to assess the tumor inhibition rate.Histological changes in the heart,spleen,liver,kidney,and lung of the mice were evaluated with HE staining,and tumor cell apoptosis was examined using TUNEL staining;The expressions of Bax,Bcl-2,GRP78,CHOP,and caspase-12 in the xenografts were detected using RT-qPCR and Western blotting.Results Cordycepin treatmentresulted in a tumor inhibition rate of 56.09%in the nude mouse models,induced obvious changes in tumor cell morphology and significantly enhanced apoptotic death of the tumor cells without causing pathological changes in the vital organs.Cordycepin treatmentalso significantly reduced Bcl-2 expression(P<0.05)and increased Bax,GRP78,CHOP,and caspase-12 expressions at both the RNA and protein levels in the tumor tissues.Conclusion Cordycepin treatment can induce apoptotic death of TCA-8113 cell xenografts in nude mice via the endogenous mitochondrial pathway and endoplasmic reticulum stress pathways.关键词
虫草素/食用菌/蛹虫草/口腔癌/细胞凋亡/异种移植物Key words
cordycepin/edible fungi/Cordyceps militaris/oral cancer/cell apoptosis/xenograft引用本文复制引用
郑庆委,邵一丹,郑婉婷,邹映雪..蛹虫草代谢产物虫草素抑制舌鳞癌裸鼠移植瘤的生长[J].南方医科大学学报,2023,43(6):873-878,6.基金项目
Supported by Key Science Research Project Funding of Education Department of Anhui Province(KJ2020A0574,KJ2018A1027)and National College Students'Innovation Training Program(202110367055).安徽省高校自然科学研究项目重点项目(KJ2020A0574、KJ2018A1027) （KJ2020A0574,KJ2018A1027）
国家级大学生创新研究项目(202110367055) （202110367055）
