中南民族大学学报（自然科学版）2023，Vol.42Issue(5)：600-608,9.DOI:10.20056/j.cnki.ZNMDZK.200230504
根癌农杆菌中DapB突变株功能的研究
Characterization of the DapB mutant of Agrobacterium tumefaciens
摘要
Abstract
Based on the amino acid sequence analysis of DapB protein(dihydrodipicolinate reductase)in Agrobacterium tumefaciens(A.tumefaciens),the dapB gene of A.tumefaciens was knocked out by transposon T5 mutation to obtain mutant and complementary strains.Compared with DapB proteins in other microorgranisms,the amino acid sequences of the agrobacterial DapB protein had the typical conserved dinucleotide binding domain and substrate binding domain.When the dapB gene was mutated by Tn5 transposon in A.tumefaciens,the growth of mutant was obviously delayed.The detention growth of mutant could restore to the level of WT by adding diaminopimelate(DAP),not by lysine.The mutant might increase the tenacity by synthesizing more protein in the membrane and enhancing the carbohydrate content in cell wall to counteract the external environment.Meanwhile,the dapB mutation influenced A.tumefaciens to response GABA.The above results showed that the DapB protein in A.tumefaciens is a functional homolog of dihydrodipicolinate reductase and directly related with the growth of cells,cell wall synthesis and cells response to GABA.This study is useful to characterize novel antibiotic target for biological control of crown gall disease caused by A.tumefaciens.关键词
根癌农杆菌/二氢吡啶二羧酸还原酶/抗生素靶位点/植物病原菌Key words
A.tumefaciens/dihydrodipicolinate reductase/antibiotic target/phytopathogen分类
生物科学引用本文复制引用
阎春兰,赵薇,陈凤美,王祖英,谭彦平..根癌农杆菌中DapB突变株功能的研究[J].中南民族大学学报（自然科学版）,2023,42(5):600-608,9.基金项目
国家自然科学基金资助项目(31772399) （31772399）
国家级大学生创新创业训练计划(202010524016) （202010524016）
