无机材料学报2023，Vol.38Issue(7)：830-838,中插9,10.DOI:10.15541/jim20220662
双相磷酸镁钙微球体外成血管和促成骨研究
In vitro Study of Biphasic Calcium Magnesium Phosphate Microspheres for Angiogenesis and Bone Formation
摘要
Abstract
Beta tricalcium phosphate(β-TCP)ceramic substituted materials have attracted a large amount of atten-tion in the last decades because of their chemical similarity with bone inorganic components,good biocompatibility,and osteoconductivity.Such materials can be used for bone replacement and bone formation in various forms,such as nanoparticles,scaffolds and microspheres.In this study,five different microsphere materials of tricalcium phosphate/trimagnesium phosphate(TMP)(TCP,25%TMP,50%TMP,75%TMP,and TMP)composites were prepared and characterized.With the increase of TMP content in the composite microspheres,the cumulative concentration of Mg2+and Ca2+released from the microspheres increased,indicating that TMP can regulate the degradation rate of the composite microspheres.The osteoblast precursor cell line(MC3T3-E1 cells)and human umbilical vein endothelial cells(HUVECs)were used as models to evaluate the biocompatibility,angiogenesis and osteogenesis of the composite microspheres.The results showed that compared with TCP,TMP and 75%TMP group,25%TMP and 50%TMP composite microspheres had better cell compatibility and had a certain proliferative effect on HUVECs.Therefore,composite microspheres of 25%TMP and 50%TMP have more significant positive effects on angiogenesis and osteogenesis.关键词
β-TCP/TMP/骨缺损/陶瓷/微球/骨组织工程Key words
β-TCP/TMP/bone defect/ceramics/microsphere/bone tissue engineering分类
通用工业技术引用本文复制引用
吴未,BAKHET Shahd,ASANTE Naomi Addai,KAREEM Shefiu,KOMBO Omar Ramadhan,李宾斌,戴红莲..双相磷酸镁钙微球体外成血管和促成骨研究[J].无机材料学报,2023,38(7):830-838,中插9,10.基金项目
National Key Research and Development Program of China(2022YFB4601402) （2022YFB4601402）
National Natural Science Foundation of China(32201109) （32201109）
Guangdong Basic and Applied Basic Research Foundation(2021A1515110557) （2021A1515110557）
Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory(XHT2020-008) （XHT2020-008）
