数字中医药（英文）2023，Vol.6Issue(2)：160-169,10.DOI:10.1016/j.dcmed.2023.07.006
黄腻苔人群舌苔与肠道菌群微生态特征及其相关性研究
Perturbations in gastrointestinal tract microbiota composition and function in individuals with yellow-greasy tongue coating
摘要
Abstract
Objective To study the composition and function of tongue coating(TC)and gastrointestinal tract(GIT)microbiota in participants with yellow-greasy tongue coating(YGTC),and to ex-plore the representative metabolite markers and pathways in this group.Methods Subjects with YGTC or thin-white tongue coating(TWTC)were recruited from December 1,2021 to October 30,2022,and the TC and fecal samples were collected.Samples were subjected to both whole-genome shotgun(WGS),and 16S rRNA gene sequencing.The α-diversity analysis,principal component analysis(PCA),and Spearman correlation analysis were performed for two groups.Ultra-performance liquid chromatography combined with tandem mass spectrometry(UPLC-MS/MS)analysis was used to analyze metabolomics and enrichment of metabolic pathways.Results The results revealed 20 YGTC participates and 19 TWTC participates.At the genus level,the dominant bacterial species of TC flora and intestinal flora in the two groups were roughly the same,but the relative kurtosis difference was marked,and the abundance of po-tentially pathogenic bacteria in TC and fecal samples of YGTC subjects was higher.There were 9 down-regulated microorganisms in the TC samples,26 down-regulated microorgan-isms,and 6 up-regulated microorganisms in YGTC subjects.The α-diversity analysis indic-ated that the Chao and abundance-based coverage estimator(ACE)indices of TC bacteria in the YGTC subjects showed a decreasing trend,but the difference was not statistically signific-ant(P>0.05).The α-diversity of fecal samples and the Chao and ACE indices decreased signi-ficantly(P<0.05).PCA showed that the microflora structure of TC and fecal samples were sig-nificantly different between the two groups.Spearman correlation analysis showed that there was no correlation between TC and fecal microorganisms at phyla and genus levels in the same subjects(P>0.05).The metabolomics results demonstrated that fumarate reductase,V/A ATPase,and phosphatidylethanolamine were increased,and glycerate-3p,UDP-glucose,and quinone oxidoreductase metabolites were decreased in YGTC TC samples.Inosine monophosphate(IMP),uridine monophosphate(UMP),and gamma-aminobutyric acid(GABA)were increased in YGTC fecal samples,while the contents of ribo-5P,histidine,biotin,and cobalamin were decreased. Metabolic pathway analysis indicated that the abundance of the TC and fecal samples of the YGTC subjects was relatively low in various metabolic path-ways, including amino acid metabolism, carbohydrate metabolism, nitrogen metabolism, and energy metabolism. Conclusion Structural and functional changes in TC and GIT microbiota or metabolite mark-ers could be potential biological bases of YGTC formation.关键词
肠道菌群/黄腻苔/微观辨证/16S rRNA/宏基因/代谢组学Key words
Gut microbiota/Yellow-greasy tongue coating/Microcosmic syndrome differentiation/16S rRNA/Metagenome/Metabonomics引用本文复制引用
刘展艳,李芷悦,朱关保,刘雅倩,彭清华,吴正治..黄腻苔人群舌苔与肠道菌群微生态特征及其相关性研究[J].数字中医药（英文）,2023,6(2):160-169,10.基金项目
China Central Finance Improvement Project for the Na-tional Key Laboratory of Traditional Chinese Medicine(China Central Finance CS[2021]No.151),the Special Key Project of Strengthening Science and Technology of Guangdong Province with Traditional Chinese Medicine(20215002),and Shenzhen Basic Research Discipline Lay-out Project(JCYJ20170412161254416). （China Central Finance CS[2021]No.151）
