数字中医药（英文）2023，Vol.6Issue(2)：170-188,19.DOI:10.1016/j.dcmed.2023.07.007
绿原酸载药类脂囊泡和前体泡囊:体外抗氧化和抗菌活性与伤口愈合疗效
Chlorogenic acid loaded niosomes and proniosomes:in vitro antioxidant and antibacterial activities with efficacy in wound healing
摘要
Abstract
Objective To develop and optimize niosomal and proniosomal vesicular delivery systems for a naturally occurring polyphenol chlorogenic acid(CGA),so as to improve its physicochemic-al properties and permeability,which may enhance its pharmacological activity.Methods The formulated CGA niosomes(CGANs)and CGA proniosomes(CGAPNs)were primed by thin film hydration and phase separation coacervation methods,and were charac-terized with different attributes including particle size,morphology,entrapment efficiency,zeta potential,deformability,in vitro diffusion,ex vivo permeability,and long-term stability.Their efficiency was further evaluated with in vitro antioxidant assay,antibacterial assays,and excision wound healing model in rats.Results Optimized CGANs and CGAPNs showed spherical vesicles with particle size of 490.1±43.0 and 280.0±22.0 nm,polydispersity index(PDI)values of 0.526 and 0.173,and stable zeta potential values of-29.3±6.4 and-33.2±6.5 mV,respectively.The CGANs and CGAPNs ves-icles showed higher entrapment(98.27%±0.46%&97.27%±0.25%)with good deformability(8.77±0.22&6.87±0.17),higher in vitro diffusion(97.96%±1.67%&91.00%±1.32%),and permeability coefficient values(67×10-3±1.72&52×10-3±1.09)with long-term stability in comparison with plain CGA.Enhanced 2-diphenyl-1-picrylhydrazyl(DPPH)radical scaven-ging and Fe2+chelation ability was obtained with CGAPNs>CGANs.They also demonstrated lethal bactericidal activity on different gram positive and gram negative strains with lower minimum inhibitory concentration(MIC)values(8×and 16×times less)as compared with plain CGA.Significant reduction(P<0.05)in wound area with higher wound contraction per-centages on day 9 was observed with CGANPs(99.56%)>CGANs(98.44%)in comparison with marketed(92.89%)and CGA(88.89%)hydrogel.Conclusion These results show great potential of CGANs and CGAPNs for topical wound healing application.This is the first study of CGA in niosomal and proniosomal topical deliv-ery systems.关键词
绿原酸/类脂囊泡/前体泡囊/抗氧化剂/抗菌/伤口愈合Key words
Chlorogenic acid/Niosomes/Proniosomes/Antioxidant/Antibacterial/Wound healing引用本文复制引用
Hemangi Ramesh Trivedi,Prashant Keshao Puranik..绿原酸载药类脂囊泡和前体泡囊:体外抗氧化和抗菌活性与伤口愈合疗效[J].数字中医药（英文）,2023,6(2):170-188,19.基金项目
The authors express their gratitude to Dr.Manoj Upad-hyay,Aston Pharmacy School and Neuroscience,Aston University for his help in carrying out confocal micro-scopy.The authors express their gratitude to Department of Pharmaceutical Sciences,Rashtrasant Tukadoji Maharaj Nagpur university for providing facilities.The authors acknowledge LIPOID GmbH Germany for providing the gift sample of LIPOID S100 for the research study. （）
