数字中医药（英文）2023，Vol.6Issue(2)：210-220,11.DOI:10.1016/j.dcmed.2023.07.010
基于网络药理学和动物实验探讨乌药治疗慢性盆腔炎的作用机制
Mechanisms of Wuyao(Linderae Radix)in treating chronic pelvic inflammatory disease based on network pharmacology and animal experiments
摘要
Abstract
Objective To predict the active components and action targets of Wuyao(Linderae Radix)in the treatment of chronic pelvic inflammatory disease(CPID)based on network pharmaco-logy,explore possible mechanisms of the treatment through animal experiments,and provide a scientific basis for clinical applications of Wuyao(Linderae Radix).Methods Possible active components and targets of Wuyao(Linderae Radix)in the treat-ment of CPID were obtained applying network pharmacology and molecular docking techno-logy.CPID rat models were established using the mixed Escherichia coli,Staphylococcus aure-us,and Ureaplasma urealyticum plus the performance of mechanical injury.Hematoxylin-eosin(HE)staining was applied to observe the pathological changes in the uterus,fallopian tube,and spleens of rat models.The contents of nitric oxide(NO),superoxide dismutase(SOD),and malondialdehyde(MDA)in the serum of rats were determined with the use of corresponding detection kits.Enzyme-linked immunosorbent assay(ELISA)test was used to measure the expression of interleukin(IL)-6 and IL-10 in the serum of rat models.Flow cyto-metry was used to determine the percentage of CD4+and CD8a+T cells as well as CD4+CD25+regulatory T cells(Tregs)in the spleen of rat models.Results A total of nine potential active components and four core therapeutic targets related to inflammatory response in Wuyao(Linderae Radix)were obtained.The animal experi-ments showed that Wuyao(Linderae Radix)markedly inhibited uterus swelling,regulated morphological changes in the fallopian tube and spleen,effectively reduced inflammatory in-filtration and injuries in the uterus and fallopian tube,and improved spleen functions in CPID rats.Moreover,Wuyao(Linderae Radix)markedly reduced the levels of NO,IL-6,and MDA,and increased the levels of IL-10 and SOD in the serum of rats.Wuyao(Linderae Radix)also elevated the percentage of CD4+T cells and the CD4+T/CD8a+T cell ratio,reduced the per-centage of CD8a+T cells,and raised the percentage of CD4+CD25+Tregs that had been ab-normally decreased in rat models(P<0.05).Conclusion Wuyao(Linderae Radix)could have therapeutic effects on CPID rats by relieving oxidative stress,mitigating inflammatory levels,and regulating the immuno-function of T cell subgroups to improve the pathological changes in CPID rats.It is a medicinal herb worth be-ing further explored for its clinical values.关键词
乌药/慢性盆腔炎/氧化应激/炎症/免疫Key words
Wuyao(Linderae Radix)/Chronic pelvic inflammatory disease/Oxidative stress/Inflammation/Immunity引用本文复制引用
谢明霞,严婧,卿栋勤,朱正清,杜可,王枭冶,王汉卿..基于网络药理学和动物实验探讨乌药治疗慢性盆腔炎的作用机制[J].数字中医药（英文）,2023,6(2):210-220,11.基金项目
Hunan Natural Science Foundation Project(2021JJ40551),Scientific Research Project of Hunan Administration of Traditional Chinese Medicine(D2022053),and Scientific Research Project of Hunan Provincial Health Commis-sion(B202313057639). （2021JJ40551）
