南方医科大学学报2023，Vol.43Issue(9)：1447-1459,13.DOI:10.12122/j.issn.1673-4254.2023.09.01
miRNA-128-3p通过下调KLHDC8A的表达抑制胶质瘤细胞的恶性生物学行为
miRNA-128-3p inhibits malignant behavior of glioma cells by downregulating KLHDC8A expression
摘要
Abstract
Objective To determine whether miRNA-128-3p regulates malignant biological behavior of glioma cells by targeting KLHDC8A.Methods Dual-luciferase reporter assays,qRT-PCR and Western blotting were used to verify the targeting of miRNA-128-3p to KLHDC8A.Edu assay,flow cytometry,Transwell assay and would healing assay were used to determine the effects of changes in miRNA-128-3p and KLHDC8A expression levels on malignant behavior of glioma cells.Rescue experiment was carried out to verify that miRNA-128-3p regulated glioma cell proliferation,apoptosis,invasion and migration by targeting KLHDC8A.Results The expression level of KLHDC8A was significantly increased in high-grade glioma tissue and was closely related to a poor survival outcome of the patients.Overexpression of KLHDC8A promoted glioma cell proliferation,migration and invasion,and miRNA-128-3p overexpression inhibited proliferative and metastatic capacities of glioma cells.Mechanistically,KLHDC8A expression was directly modulated by miRNA-128-3p,which,by targeting KLHDC8A,inhibited malignant behavior of glioma cells.Conclusion Upregulation of miRNA-128-3p inhibits uncontrolled growth of glioma cells by negatively regulating KLHDC8A expression and its downstream effectors,suggesting that the miRNA-128-3p-KLHDC8A axis may serve as a potential prognostic indicator and a therapeutic target for developing new strategies for glioma treatment.关键词
胶质瘤/miRNA-128-3p/KLHDC8A/生物标志物/迁移/增殖Key words
glioma/miRNA-128-3p/KLHDC8A/biomarker/migration/proliferation引用本文复制引用
于正涛,李佳梦,蒋俊文,李由,林珑,夏鹰,王磊..miRNA-128-3p通过下调KLHDC8A的表达抑制胶质瘤细胞的恶性生物学行为[J].南方医科大学学报,2023,43(9):1447-1459,13.基金项目
Supported by Hainan Provincial Natural Science Foundation of China(No.820MS163),Scientific Research Project of Hunan Provincial Health Commission(No.20200709),and Hainan Cerebrovascular Diseases Clinical Medical Research Center(LCYX202206). 基金项目:海南省自然科学基金(820MS163) （No.820MS163）
湖南省卫生健康委员会科研项目(20200709) （20200709）
海南省脑血管病临床医学研究中心资助项目(LCYX202206) （LCYX202206）
