数字中医药（英文）2023，Vol.6Issue(3)：272-284,13.DOI:10.1016/j.dcmed.2023.10.003
基于质量源于设计理念研究不同地区余甘子的关键性状
Quality by Design approach for the investigation of critical characteristics of Phyllanthus emblica from different vicinities
Grishm Rohilla 1Priya Masand 2Pooja Dhama 1Anurag 2Sunil Gupta 3Alok Sharma2
作者信息
- 1. Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab 142001, India
- 2. Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology, Meerut, Uttar Pradesh 250005, India
- 3. School of Pharmacy, Mangalayatan University, Aligarh, Uttar Pradesh 202146, India
- 折叠
摘要
Abstract
Objective To explore the application of Quality by Design(QbD)tools in assessing geograph-ical variations of Phyllanthus emblica(P.emblica)from five distinct Indian states.
Methods In the current experiment,the Box-Behnken design with a reduced quartic model and 105 runs was employed with the use of the Design Expert software for randomized re-sponse surface mapping.Three different extraction methods(Soxhlet,maceration,and soni-cation)along with three solventst[distilled water,methanol,and water-methanol mixture(50:50 v/v)]were considered in the present study.The anti-oxidant activities,total flavonoid content(TFC),and total phenolic content(TPC)in the P.emblica were determined and anal-ysed by gas chromatography-mass spectrometry(GC-MS)to identify the major components.
Results The QbD overlay plot showed that the extractive value of the P.emblica was no less than 30%w/w,2,2-diphenyl-1-picrylhydrazyl(DPPH)no less than 60%mcg/mL(micrograms per millilitre),TFC no less than 75 mg QE/g(milligrams of quercetin equivalents per gram),and TPC no less than 80 mg GAE/g(milligrams of gallic acid equivalents per gram).Moreover,the GC-MS data confirmed the presence of variation in the bioactives of P.embli-ca extracts.
Conclusion The model was significant in describing the variation in extractive value,DPPH,TFC,and TPC.The QbD approach may tend to prioritize thoroughness in the extraction pro-cess,ultimately resulting in improved quality in the extracted products.关键词
余甘子/质量源于设计/气相色谱-质谱法/浸提参数/生物活性Key words
Phyllanthus emblica/Quality by Design(QbD)/Gas chromatography-mass spectrome-try(GC-MS)/Extraction parameters/Bioactives引用本文复制引用
Grishm Rohilla,Priya Masand,Pooja Dhama,Anurag,Sunil Gupta,Alok Sharma..基于质量源于设计理念研究不同地区余甘子的关键性状[J].数字中医药（英文）,2023,6(3):272-284,13.
