遵义医科大学学报2023，Vol.46Issue(11)：1076-1087,12.
多囊卵巢综合征患者血清微囊泡microRNAs的研究
Serum microvesicle microRNAs in patients with polycystic ovary syndrome
摘要
Abstract
Objective To detect serum microvesicles(MVs)concentrations and expression profiles of microvesi-cle microRNAs(miRNAs)in patients with polycystic ovary syndrome(PCOS)and control groups,and analyze their potential role in the pathogenesis of PCOS.Methods Fifty serum samples from PCOS patients and 50 age-matched serum samples from women seeking fertility treatment as controls were collected.MVs were isolated through differential centrifugation and characterized by transmission electron microscopy(TEM),Western blot,and nanoparticle tracking analysis(NTA).The differentially expressed miRNAs were screened by high-through-put sequencing methods,and the targets of these miRNAs were predicted.GO/KEGG pathway enrichment analysis were performed.Female ICR mice were injected with serum MVs suspension from PCOS patients to ob-serve the estrus cycle and ovarian structure.Immunohistochemical methods were used to detect the expression and localization of NF-κB and WNT5A proteins related to PCOS inflammation in the ovary.Results We found that serum MVs levels tended to be higher in the PCOS group and were dominated by small-diameter vesicles.High-throughput sequencing identified 19 differentially expressed miRNAs,of which 8 were up-regulated and 11 were down-regulated(P＜0.05).Five differentially expressed miRNAs were identified by qRT-PCR,mong which three miRNAs were identical with the sequencing results.Enrichment analysis revealed that possible path-ways associated with PCOS include AGE-RAGE signaling pathway,MAPK signaling pathway,and TGF-β signa-ling pathways.Injection of PCOS serum MVs caused estrous cycle disturbance in female ICR mice,and inflam-mation related protein NF-κB was positively expressed in the nucleus.Conclusion Small diameter MVs were more abundant in the serum of PCOS patients,and the differentially expressed miRNAs may be involved in multi-ple PCOS-related pathways.PCOS serum MVs can induce a mild PCOS-like phenotype in female mice.关键词
微囊泡/多囊卵巢综合征/miRNA/卵泡发育Key words
microvesicles/polycystic ovary syndrome/miRNA/follicular development分类
医药卫生引用本文复制引用
潘茂美,文婷媛,蒋君,张媚,王德婧..多囊卵巢综合征患者血清微囊泡microRNAs的研究[J].遵义医科大学学报,2023,46(11):1076-1087,12.基金项目
贵州省科技厅基金资助项目[NO:(2017)7104] （2017）
遵义医科大学博士启动基金资助项目(NO:201704). （NO:201704）
