大豆科学2023，Vol.42Issue(6)：641-652,12.DOI:10.11861/j.issn.1000-9841.2023.06.0641
野生大豆×栽培大豆RIL群体高密度遗传图谱构建及SNP偏分离分析
High Density Genetic Map Construction and SNP Segregation Distortion Analysis in RIL Population of Wild Soybean x Cultivated Soybean
摘要
Abstract
This study examined the segregation distortion during the process of constructing wild soybean and cultivated soybean population,to explore the segregation distortion regions(SDRs)and candidate genes,and to shed some light on understanding the mechanism of segregation distortion in soybean.The wild variety'Changling wild soybean'was used as the male parent and the landrace variety'Yiqianli'was used as the female parent to develop a hybrid group,resulting in 200 recombinant inbred lines(RIL).SLAF-seq was used for sequencing analysis and constructing a high-density genetic map.4 564 SNP markers were obtained and reliably identified for this population.Through segregation distortion analysis,648 markers were found to have genetic distortion(P＜0.05),accounting for 14.20％of the total markers.22 SDRs were found,which were distributed across 9 different chromosomes.In SDRs,8 extreme SDRs regions(ESDRs)were found,distributed on 5 different chromosomes,of which 3 ESDRs were biased towards the wild type of the male parent,and 5 ESDRs were biased towards the cultured type of the female parent.Using gene function annotation and genome-wide resequencing data,combined with the ESDR region,the affecting embryonic development(Glyma.01G051400)and female gametophyte development(Glyma.16G072700)genes were identified as candidate genes in ESDR1-1 and ESDR16-1,respectively.This study provides a reliable basis for locating the segregation distortion genes in the future,and lays the foundation for elucidating the segregation distortion.关键词
栽培大豆/野生大豆/偏分离/SNP/重组自交系Key words
cultivated soybean/wild soybean/partial segregation/SNP/recombinant inbred line引用本文复制引用
刘德泉,聂波涛,邱红梅,陈亮,陈健,崔正果,姬文秀,王跃强..野生大豆×栽培大豆RIL群体高密度遗传图谱构建及SNP偏分离分析[J].大豆科学,2023,42(6):641-652,12.基金项目
吉林省农业科技创新工程(CXGC2022RCG005,CXGC2022RCY008). （CXGC2022RCG005,CXGC2022RCY008）
