大豆科学2023，Vol.42Issue(6)：674-682,9.DOI:10.11861/j.issn.1000-9841.2023.06.0674
大豆盐胁迫响应NRT基因的鉴定及GmNRT1.5A的克隆和表达分析
Identification of Soybean Salt Stress Responsive NRT Genes and Cloning and Expression Analysis of GmNRT1.5A
摘要
Abstract
Nitrate transporters(NRTs)play an important role in NO3-uptake or transporters in plant roots.In order to study the function of soybean NRTs genes in response to salt stress,eight differentially expressed GmNRTs genes that respond to salt stress were identified from the transcriptome data of salt stress treatment.The promoters of these GmNRTs genes contain multiple plant stress or plant hormones response elements,suggesting that these genes might be involved in abiotic stress or plant hormone regulation of salt stress response of soybean.At the same time,we cloned GmNRT1.5A from soybean roots,which was the most up-regulated gene under salt stress treatment.The GmNRT1.5A CDs of 1 794 bp in length,encoded a 597 amino acid,with molecular weight of 66.78 kD and isoelectric point(pI)of 5.85.Phylogenetic analysis revealed that GmNRT1.5A had a close relationship with AtNRT1.5 and ZmNRT2.5,contained twelve transmembrane domains and typical NRT1s conserved domains,and the secondary structure of GmNRT1.5A was found to be composed of α-helix and random coils.Real-time quantitative PCR analysis showed that GmNRT1.5A was mainly expressed in seeds and roots.Salt stress and drought stress significantly induced the expression of GmNRT1.5A gene in root.In addition,the expression of GmNRT1.5A was significantly induced by ABA and ACC,while GA3 treatment significantly inhibited the expression of GmNRT1.5 A.This study showed that GmNRT1.5A played an important role in response to salt stress,which provides a new idea for exploring the function of NRTs genes in soybean response to stress,and also lays a foundation for in-depth research on the function of GmNRT1.5A gene in soybean.关键词
大豆/盐胁迫/NRTs/GmNRT1.5A/表达分析Key words
soybean/salt stress/NRTs/GmNRT1.5A/expression analysis引用本文复制引用
王玉斌,张志贤,张辰淼,刘薇,张彦威,张礼凤,徐冉..大豆盐胁迫响应NRT基因的鉴定及GmNRT1.5A的克隆和表达分析[J].大豆科学,2023,42(6):674-682,9.基金项目
国家自然科学基金重点项目(U22A20467) （U22A20467）
山东省自然科学青年基金项目(ZR2020MC101) （ZR2020MC101）
山东省农业科学院科技创新工程项目(cxgx2023A02) （cxgx2023A02）
国家体系济南试验站项目(CARS-04-CES12). （CARS-04-CES12）
