动物医学进展2023，Vol.44Issue(12)：1-5,5.
内蒙古某奶牛场两种致犊牛腹泻原虫的鉴定及遗传进化分析
Identification and Phylogenetic Analysis of Two Intestinal Protozoa from a Dairy Farm in Inner Mongolia
摘要
Abstract
To investigate the infection and molecular characteristics of important intestinal protozoa causing calf diarrhea of a dairy farm in Inner Mongolia,in this study,18 fresh feces samples of calves with diarrhea were collected to isolate and identify Giardia lamblia and Cryptosporidium sp.in the samples based on morphological and molecular biological methods.The results of microscopic observation on morphology showed that the infection rates of Giardia lamblia and Cryptosporidium sp.were 72.22％(13/18)and 5.56％(1/18),respectively.Furtherly,total DNA preparation was extracted from 18 fecal samples and used for PCR amplification,sequence comparison and phylogenetic analysis on the BG and SSU rRNA gene se-quences of Giardia lamblia and Cryptosporidium sp.Electrophoresis results showed that the purpose stripes were detected at 830 bp and 511 bp in microscopically positive fecal samples.Meanwhile,sequence comparison and phylogenetic analysis also suggested that all isolations of Giardia lamblia here belonged to assemblage E due to possession of 99.78％sequence similarity in BG gene locus with assemblage E refe-rence isolates retrieved from GenBank.And the only Cryptosporidium sp.isolate in the study,shared 100％sequence similarity on SSU rRNA gene locus with Cryptosporidium bovis from different countries and ar-eas,was grouped into the same evolutionary branch.In this study,C.bovis and Giardia lamblia were iden-tified as intestinal parasitic protozoa causing calf diarrhea.The results can provide theoretical basis for the prevention and treatment of calf diarrhea in this area.关键词
犊牛/腹泻/蓝氏贾第虫/牛隐孢子虫/鉴定Key words
calf/diarrhea/Giardia lamblia/Cryptosporidium bovis/identification分类
农业科技引用本文复制引用
刘鼎,梁高星,房凯敏,任尊,牛雨薇,吴敏楠,宋军科..内蒙古某奶牛场两种致犊牛腹泻原虫的鉴定及遗传进化分析[J].动物医学进展,2023,44(12):1-5,5.基金项目
陕西省重点研发计划项目(2020NY-006) （2020NY-006）
