动物医学进展2023，Vol.44Issue(12)：6-9,4.
猪繁殖与呼吸综合征两种减毒活疫苗免疫后病毒载量及抗体水平分析
Analyse of Virus Loads and Antibody Levels after Immunization with Two PRRSV Live Attenuated Vaccines
摘要
Abstract
In order to understand the differences in viremia and antibody levels in pigs immunized with live attenuated commercial porcine reproductive and respiratory syndrome vaccines A and B,100 35 day old re-serve sows were randomly selected from the reserve sow farm and divided into groups A and B.After grouping,the pigs were mixed bred for 14 days.At the age of 50 days,50 reserve sows in group A were im-munized with vaccine A,and 50 reserve sows in group B were immunized with vaccine B(1 portion per head).On days 0,1,2,3,5,7,10,14,21,28,and 35 of vaccine immunization,10 samples of sera were ran-domly collected and isolated from pigs in each experimental group.The viral load of porcine reproductive and respiratory syndrome was detected by fluorescence quantitative PCR,and the antibody to porcine re-productive and respiratory syndrome virus was detected by ELISA.The results showed that:After immuni-zation with B vaccine,the duration of the experimental porcine reproductive and respiratory syndrome vire-mia period was longer,lasting up to 35 days;After immunization with vaccine A,the experimental porcine reproductive and respiratory syndrome virus load was higher;After immunization with B vaccine,the varia-tion trend of N-protein antibody level in pigs was more stable;After immunization with B vaccine,the level of GP protein antibody in pigs was higher.This indicates that the immune effect of vaccine B is better than that of vaccine A.关键词
猪繁殖与呼吸综合征/减毒活疫苗/病毒血症/抗体衰减Key words
Porcine reproductive and respiratory syndrome/attenuated live vaccine/viremia/antibody attenuation分类
农业科技引用本文复制引用
周宏超,贾佩,许信刚..猪繁殖与呼吸综合征两种减毒活疫苗免疫后病毒载量及抗体水平分析[J].动物医学进展,2023,44(12):6-9,4.基金项目
陕西省农业农村厅省级农业专项项目(2019SJNYZX45) （2019SJNYZX45）
