动物医学进展2023，Vol.44Issue(12)：10-16,7.
鸡肉加工及零售环节中分离的沙门氏菌血清型、毒力基因及耐药性检测
Detection of Serotype,Virulence Gene and Drug Resistance of Salmonella in Chicken Processing and Retail Links
摘要
Abstract
To study the pathogenicity,serotype distribution,virulence gene and drug resistance of Salmonel-la in chicken processing and retail links in Chengde,Hebei province,in this experiment,257 samples from chicken slaughterhouses,processing plants and retail stores in Chengde,Hebei province were collected from 2021-2022 for isolation and identification of Salmonella.The pathogenicity,serotype,virulence gene and drug resistance of the isolated strains were detected by artificial infection mouse test,glass plate agglutina-tion test,PCR method and K-B drug sensitive paper method.The results showed that 70 pathogenic Salmo-nella strains were isolated from 257 samples;There are 12 serotypes in 70 strains of Salmonella,among which Salmonella enteritidis(24.3％),Salmonella typhimurium(21.4％)and Salmonella dublin(17.1％)are the dominant serotypes;The detection rates of virulence plasmid genes(spvA,spvB,spvC,spvR)were between 12.9％and 88.6％,and the detection rates of virulence island genes(SPI-1,SPI-2,SPI-3,SPI-5)were between 27.0％and 90.0％;The drug resistance rate to 7 drugs,such as amoxicillin,ampicillin and sulfamethoxypyrimidine,was more than 51.4％,and the drug resistance rate to other drugs was between 5.7％and 40％,with 10(15.0％),9(20.0％)and 8(17.1％)drugs being the main resistance;The detection rate of drug resistance genes Sul1,Sul2,Sul3,TetA,TetM,TetR,aac(3)-Ⅰ v and aac(6')-Ⅰb was 45.7％-97.1％,and the detection rate of other drug resistance genes was between 8.6％and 17.1％.There was basically positive correlation between drug resistance phenotype and drug resistance genes(except amidohols and polymyxin).The results showed that 70 strains of pathogenic Salmonella iso-lated from chicken processing and retail links in Chengde area had multiple serotypes,carried multiple viru-lence genes,serious drug resistance,and carried multiple drug resistance genes.关键词
鸡肉/沙门氏菌/致病性/血清型/毒力基因/耐药基因Key words
chicken/Salmonella/pathogenicity/serotype/virulence gene/drug resistance gene分类
农业科技引用本文复制引用
邱青璐,王铄,张召兴,刘冬,崔海明,张艳英..鸡肉加工及零售环节中分离的沙门氏菌血清型、毒力基因及耐药性检测[J].动物医学进展,2023,44(12):10-16,7.基金项目
河北省大中学生科技创新能力培育专项项目(22E50287D) （22E50287D）
承德市科学技术局承德市绿色食品产业研究院建设项目(202205B088) （202205B088）
